Noninvasive Fluorescence Monitoring
Noninvasive Fluorescence Monitoring - Related Videos
09:31
Noninvasive Blood Pressure Measurement Techniques
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2023
Source: Hamna J. Qureshi and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana Here we will highlight the key similarities and differences of noninvasive blood pressure measurement techniques between humans and rodents and examine the engineering principles that govern blood pressure. The principles that govern current cuff technology to acquire systolic and diastolic pressures will also be discussed. Commercially available cuffs that connect...
08:16
Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis
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Cited by 10 •
2019
Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real...
07:48
Noninvasive and Invasive Renal Hypoxia Monitoring in a Porcine Model of Hemorrhagic Shock
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Cited by 4 •
2022
Presented here is a protocol to measure renal oxygenation in the medulla and noninvasive urine oxygen partial pressure in a hemorrhagic shock porcine model to establish urine oxygen partial pressure as an early indicator of acute kidney injury (AKI) and a novel resuscitative endpoint.
03:36
Fluorescence-Based Monitoring of Mitochondrial Stress in Astrocytes
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2025
This video demonstrates the evaluation of mitochondrial health in genetically modified astrocytes, which express a fluorescent protein within their mitochondria. The protein helps to track changes in mitochondrial health as its fluorescence changes from green to red, indicating stress or damage. Images are captured and analyzed using various parameters.
10:04
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
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Cited by 4 •
2014
Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.
FAQs
A fluorophore or genetically encoded reporter first absorbs excitation light and then emits light at a longer wavelength. External detectors collect that emitted signal, allowing researchers to distinguish the fluorescent response from the incoming illumination. Changes in signal location or activity can therefore be followed as indicators of labeled cells, molecules, or physiological processes within a living system.
The measured signal can provide information about where labeled cells or molecules are located and how reporter activity changes over time. This makes the method useful for tracking biological processes rather than relying only on a single endpoint observation. Interpretation depends on what the selected fluorophore or genetically encoded reporter represents in the experimental system.
Repeated measurements from the same living system improve temporal analysis because researchers can follow changes across time without removing tissue at each observation. This longitudinal design can reduce sampling damage and reveal patterns in gene expression, cell behavior, disease progression, or treatment response that may be difficult to reconstruct from separate endpoint samples.
A typical workflow begins by establishing a fluorescent label or reporter that corresponds to the biological feature under study. The living system is exposed to excitation light, and external detectors collect the emitted signal. Researchers then compare signal location or activity across observations to assess changes in the labeled cells, molecules, or physiological process.
This approach is especially useful when the study requires observations from the same living system over multiple time points. Unlike endpoint microscopy, which contributes information after a terminal observation, repeated external detection supports longitudinal analysis of disease progression, treatment responses, gene expression, or cell behavior. It can also complement endpoint microscopy rather than replace it.
In biology, the method can follow gene expression, cell behavior, disease progression, and responses to treatment while limiting disruption caused by tissue removal. Its value comes from combining spatial information about labeled targets with repeated observations over time. Researchers can use these measurements alongside invasive assays and endpoint microscopy to broaden interpretation of biological changes.