Optical Parametric Oscillator

An optical parametric oscillator (OPO) is a tunable light source that converts laser energy into new wavelengths, enabling precise illumination and detection across broad regions of the electromagnetic spectrum. It works when a high-intensity pump beam passes through a nonlinear optical crystal, where parametric amplification produces two lower-energy fields, called the signal and idler; optical feedback from a resonant cavity sustains oscillation when phase-matching conditions are met. In biology, OPOs support fluorescence spectroscopy, vibrational imaging, and nonlinear microscopy by providing adjustable infrared or visible excitation. Their wavelength flexibility improves molecular contrast and helps researchers study cellular composition, tissue structure, and dynamic biological processes.

Optical Parametric Oscillator - Related Videos

Research

JoVE Journal - Engineering

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

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Cited by 13 •

2014

We describe the reliable generation of non-Gaussian states of traveling optical fields, including single-photon states and coherent state superpositions, using a conditional preparation method operated on the non-classical light emitted by optical parametric oscillators. Type-I and type-II phase-matched oscillators are considered and common procedures, such as the required frequency filtering or the high-efficiency quantum state characterization by homodyning, are detailed.

Fabrication and Testing of Microfluidic Optomechanical Oscillators

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Cited by 10 •

2014

Parametric optomechanical excitations have recently been experimentally demonstrated in microfluidic optomechanical resonators by means of optical radiation pressure and stimulated Brillouin scattering. This paper describes the fabrication of these microfluidic resonators along with methodologies for generating and verifying optomechanical oscillations.

Selective Manipulation of Theta Oscillations in a Mouse Model

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2025

This video demonstrates a protocol to modulate theta oscillations in a genetically engineered mouse model with light-sensitive ion channels in its GABAergic medial septum neurons. By delivering light pulses to the hippocampus, the protocol aims to synchronize neuronal activity and generate theta oscillations, which are recorded as local field potentials.

Education

JoVE Core - Calculus

Parametric Surfaces

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2026

A parametric surface in three-dimensional space is defined through a vector-valued function\begin{equation*}\mathbf{r}(u, v) = x(u, v)\mathbf{i} + y(u, v)\mathbf{j} + z(u, v)\mathbf{k}\end{equation*}where u and v are parameters within a specified domain D in the uv-plane. The functions x(u, v), y(u, v), and z(u, v) define the coordinates of points on the surface. As u and v vary over D, the position vector r(u, v) traces a continuous surface in space. This parametric representation is essential...

In Vitro Recording of Theta Oscillations in the Mouse Hippocampus

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2025

This video demonstrates the recording of rhythmic neuronal network activity, also known as theta oscillations, in an isolated hippocampus. Electrodes are inserted into various layers and spatial positions to detect and analyze theta oscillations. This shows the unique neuronal compositions and the connectivity of the neuronal network across different layers of the hippocampus.

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