Particle Extraction
Particle Extraction - Related Videos
10:37
Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts
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Cited by 6 •
2016
The design-of-experiments procedure presented here allows the evaluation of different flocculants in terms of their ability to aggregate dispersed particles in plant extracts, thus reducing turbidity and the costs of downstream processing.
03:43
A Technique for Magnetic Glass Particle-Mediated Nucleic Acid Extraction from Blood
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2025
This video demonstrates a technique for nucleic acid extraction from whole human blood using magnetic glass particles (MGPs) — small magnetic beads coated with silica. Upon lysing the blood cells using a lysis buffer, the released nucleic acids bind to the surface of silica-coated MGPs, extracting the molecules from the sample.
03:37
Subatomic Particles
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2020
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom. The Discovery of the Electron The first clue about the subatomic structure came at the end of the 19th century when J.J. Thomson discovered the electron using a cathode ray tube. This apparatus consisted of a sealed glass...
02:55
Extraction - Concepts
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2020
Extraction Extraction is a common technique used in organic chemistry to isolate a target compound. In the extraction process, a solute is transferred from one phase to another to separate it from unreacted starting materials or impurities. Extraction is also used to facilitate the isolation of a solute from a reaction solvent that is difficult to remove by evaporation, such as a solvent with a high boiling point. Generally, there are three types of extractions. First, in solid-liquid...
02:41
Concentration and Purification of Viral Particles
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2026
Source: Fricano-Kugler, C. J., et al. Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection. J. Vis. Exp. (2016).This video demonstrates the step-by-step process for concentrating and purifying lentiviral particles from a mixed suspension containing host cell debris. The procedure utilizes centrifugation, filtration, and PEG-salt precipitation to isolate viral particles while removing soluble impurities. The final product is resuspended in a...
FAQs
The central design choice in Particle Extraction is matching the separation principle to the difference between the target particles and their surrounding material. Size differences can support filtration, while density-related differences can guide centrifugation or sedimentation. Shape and surface properties provide additional selection criteria, allowing the same biological sample to be approached in different ways.
Filtration, centrifugation, sedimentation, and selective binding do not separate particles for the same reason. Filtration emphasizes passage through a barrier, centrifugation and sedimentation exploit physical differences that influence how components move or settle, and selective binding uses surface properties. Choosing among them depends on which distinguishing feature best separates the desired material from its surroundings.
Selective binding is useful when the desired particles differ from surrounding material in surface properties. A binding-based approach can therefore favor recovery of particles with the relevant surface characteristics rather than relying primarily on size, density, or shape. In biological work, this provides a complementary strategy for isolating particular cells, organelles, extracellular vesicles, or microorganisms.
Extraction quality matters because the isolated fraction becomes the input for later analysis. Inefficient separation can limit the material available for imaging, molecular analysis, particle counting, or diagnostic testing, whereas effective extraction supports more reliable downstream results. The extraction method is therefore part of experimental quality control, not merely a preliminary handling step.
A practical workflow begins by identifying the particulate material of interest and the property that distinguishes it from the surrounding sample. The researcher then selects a compatible approach, such as filtration, centrifugation, sedimentation, or selective binding, and recovers the separated material for identification, measurement, or further study. This links method selection directly to the intended outcome.
Particle Extraction supports several biological investigations because the recovered material can include cells, organelles, extracellular vesicles, microorganisms, and other particulate material from fluids or tissues. After separation, these fractions may be examined by imaging, molecular analysis, particle counting, or diagnostic testing. The method is especially relevant when complex samples contain multiple particulate components requiring focused study.