Quarantine Biosecurity
Quarantine Biosecurity - Related Videos
09:58
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems
0 Views •
Cited by 17 •
2017
This protocol describes the use of sump swabs and sludge analysis of zebrafish systems, which leads to increased detection compared to the sole use of sentinels to detect pathogens such as Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa. A system to monitor P. tomentosa eggs in quarantine is also proposed.
11:39
Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
0 Views •
Cited by 39 •
2012
Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.
09:32
Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
0 Views •
Cited by 1 •
2019
Here, we present a protocol for collection of confocal Raman spectra from human subjects in clinical studies combined with chemometric approaches for spectral outlier removal and the subsequent extraction of key features.
11:56
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
0 Views •
Cited by 12 •
2017
An in vitro bioluminescence assay to determine cellular circadian rhythm in mammary epithelial cells is presented. This method utilizes mammalian cell reporter plasmids expressing destabilized luciferase under the control of the PERIOD 2 gene promoter. It can be adapted to other cell types to evaluate organ-specific effects on circadian rhythm.
11:36
Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs
0 Views •
Cited by 40 •
2012
Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.
FAQs
The central control is separation: newly introduced or potentially exposed organisms remain apart from established populations while access and movement are restricted. This reduces opportunities for an infectious agent or pest to pass through direct or indirect contact. Applying the same controls to people, animals, materials, and equipment helps prevent transmission from crossing the quarantine boundary.
Monitoring during a defined observation period provides time to identify infection before an organism contacts an established population. Sanitation and decontamination add a second safeguard by addressing biological hazards that may remain on materials or equipment. Together, observation and cleaning help detect threats early and reduce the chance that undetected hazards spread.
Controls should address the movement of people, animals, materials, and equipment rather than focusing only on the organisms being introduced. Any of these pathways can connect a potentially exposed organism or biological hazard with an established population or facility. Restricting movement across these routes strengthens containment and supports more reliable monitoring.
A basic procedure begins by separating newly introduced or potentially exposed organisms from established populations. It then restricts movement of people, animals, materials, and equipment, followed by monitoring throughout a defined observation period. Sanitation and decontamination measures are applied during this period to reduce biological hazards and limit their spread within or beyond the facility.
Quarantine biosecurity supports work in laboratories, animal facilities, agriculture, aquaculture, and wildlife management. The specific organisms and hazards differ across these settings, but each may require protection from introduced infectious agents, pests, or other biological threats. Its broad use reflects the need to protect research systems, managed populations, crops, aquatic organisms, and wildlife.
Effective practices can help identify infection before contact with established populations, reduce transmission, and protect animal and plant health. In research settings, they also support safer biological work. At a broader level, limiting the spread of infectious agents and pests can reduce the ecological and economic consequences associated with biological disease outbreaks.