Pcr Fingerprinting
Pcr Fingerprinting - Related Videos
09:39
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
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Cited by 9 •
2011
Leprosy, caused by Mycobacterium leprae, is still endemic in many places. In order to learn about the spread and mode of transmission of leprosy, it is important to determine which strain of M. leprae has infected a patient. Variable numbers of tandem repeats (VNTR) typing is one such method.
12:02
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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Cited by 18 •
2016
We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.
01:03
IR Frequency Region: Fingerprint Region
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2024
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1. The...
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Cited by 9 •
2016
Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.
03:41
Agarose Gel Electrophoresis of DNA Amplicons Post PCR: A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success
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2025
In this video, we demonstrate the separation of bacterial PCR-amplified DNA using agarose gel electrophoresis. Agarose gel functions as a molecular sieve, enabling the negatively-charged DNA to migrate based upon their size under an applied electric field.
FAQs
The primers determine which genomic regions become amplified. When primer-binding sites differ among samples, PCR produces fragments with different lengths or combinations, creating distinct banding patterns after separation. Using primers that recognize multiple sites can survey variation across several regions at once, making the resulting profile useful for comparing biological samples.
Gel electrophoresis separates the amplified DNA fragments according to their size, allowing researchers to visualize a sample’s banding pattern. The relative presence, absence, and arrangement of bands provide the comparison framework. Similar profiles suggest shared genetic features, whereas clearly different patterns can reveal variation between species, strains, cell lines, or individuals.
PCR fingerprinting compares patterns of amplified fragments rather than determining the complete nucleotide sequence of a genome. This makes it a relatively rapid and accessible approach when researchers need to distinguish samples or assess relatedness without characterizing every base. Sequencing provides more detailed genetic information, while fingerprinting emphasizes efficient profile-level comparison.
The regions selected for amplification shape the information contained in the profile. Primers may target selected genomic regions or broadly distributed regions, so different primer designs can reveal different aspects of variation. Researchers therefore interpret a profile in relation to the genomic areas sampled rather than treating one banding pattern as a complete description of the genome.
A typical workflow begins with DNA samples and PCR amplification using primers designed for selected or broadly distributed genomic regions. The amplified products are then separated by gel electrophoresis, which resolves fragments into visible bands. Researchers compare the resulting profiles across samples to identify matching, differing, or unexpectedly mixed patterns.
The method is useful when researchers need a practical comparison of genetic variation among species, strains, cell lines, or individuals. It can support assessments of genetic relatedness and help detect contamination or sample mix-ups. Its value is greatest when a distinctive comparative profile is sufficient and complete DNA sequencing is unnecessary.