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DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) – Fragment Length Analysis (FLA)
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) – Fragment Length Analysis (FLA)

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) – Fragment Length Analysis (FLA)

DOI:
10.3791/3104-v

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09:39 min

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July 15, 2011

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, , ,
1Department of Microbiology, Immunology and Pathology,Colorado State University

Chapters

  • 00:05Title
  • 01:39Amplification of Bacterial DNA Using Multiplex PCR
  • 04:05Fragment Length Analysis: Preparation of Samples and Electrophoresis
  • 06:53Analysis of Fragment Length Results
  • 08:24Fragment Length Analysis for Leprosy Patients in the Philippines
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

Leprosy, caused by Mycobacterium leprae, is still endemic in many places. In order to learn about the spread and mode of transmission of leprosy, it is important to determine which strain of M. leprae has infected a patient. Variable numbers of tandem repeats (VNTR) typing is one such method.

Tags

DNA FingerprintingMycobacterium Leprae StrainsVariable Number Tandem Repeat (VNTR)Fragment Length Analysis (FLA)Leprosy TransmissionLeprosy PatientsArmadillosNude MiceEpidemiology MethodsGlobal Drug Treatment ProgramLeprosy CasesM. Leprae Genome MappingMicro- And MinisatellitesShort Tandem Repeats (STR)Variable Number Tandem Repeat (VNTR) AnalysisStrain Typing

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