Plant Microbe Interactions

Plant microbe interactions are relationships between plants and microorganisms, including bacteria and fungi, that influence plant growth, health, and survival. They are mediated by molecular signals and chemical exchanges at sites such as the root surface, where microbes may alter nutrient availability, produce growth-promoting compounds, or trigger plant immune responses. The outcome depends on environmental conditions and the partners involved, ranging from mutualistic associations that improve nutrient acquisition to infections that cause disease. Studying these interactions supports research in plant biology and can inform sustainable agriculture, including the use of beneficial microbes to improve crop productivity, stress tolerance, and soil health.

Plant Microbe Interactions - Related Videos

Education

JoVE Core - Microbiology

Microbe-Plant Interactions

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2026

Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...

Research

JoVE EoE - Bacterial Growth and Techniques

Treatment of Bacteria with Plant Root Exudates to Emulate In Vitro Plant-Microbe Interactions

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2025

Source: Yi, Y., et al. Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates. J. Vis. Exp. (2018)This video demonstrates the preparation and treatment of Bacillus mycoides cultures to study plant-microbe interactions. It outlines the steps from streaking a stock culture and incubating it in liquid medium to exposing the bacteria to plant root exudates under agitation. The process culminates in harvesting and flash-freezing the bacterial cells for...

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

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Cited by 6 •

2017

The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be investigated.

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

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Cited by 32 •

2016

We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein tag.

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

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Cited by 4 •

2018

The goal of the protocol presented here is to study the transcriptomic response of endosphere-isolated Bacillus mycoides to potato root exudates. This method facilitates the identification of important bacterial genes involved in plant-microbe interactions and is in principle applicable to other endophytes and plants, with minor adjustments.

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