Ppe Usage
Ppe Usage - Related Videos
11:02
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
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Cited by 1 •
2016
With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.
01:22
PPE Use in Healthcare Settings I: Donning
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2023
Donning PPE must be completed before contact with the patient. This process protects from infectious agents. The sequence and action included in each donning are critical, and the steps must be systematic to avoid exposure to pathogens. The institutional policy also needs to be followed while donning PPE. The pre-donning preparations are gathering equipment, inspecting the PPE equipment for tears, holes, or damage, removing jewelry, removing any garments below the elbows, and tying the hair...
01:10
PPE Use in Healthcare Settings II: Doffing
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2023
The sequence of removing or doffing PPE starts with the gloves, as they are the most contaminated. Next is removal of the face shield or goggles, as they would interfere with removing other PPE. Then remove the gown, followed by the mask or respirator. Perform hand hygiene between steps if hands become contaminated and immediately after removing all PPE. Generally, the outside front and sleeves of the isolation gown, the goggles or the mask, the respirator, and the face shield are contaminated.
07:06
Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach
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Cited by 16 •
2011
The prediction of the coreceptor usage of HIV-1 is required for the administration of a new class of antiretroviral drugs, i.e. coreceptor antagonists. It can be performed by sequence analysis of the env gene and subsequent interpretation through an internet based interpretation system (geno2pheno[coreceptor]).
10:22
MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
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Cited by 3 •
2021
A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.
FAQs
Selection should reflect the exposure being controlled rather than rely on a single standard combination of equipment. Biological work may involve infectious materials, splashes, aerosols, or contaminated surfaces, while chemical and physical hazards may require additional protection. Matching gloves, laboratory coats, eye protection, masks, or face shields to the anticipated exposure helps create an appropriate protective barrier.
Protective equipment works only when it remains properly positioned and is handled without transferring contamination. An unsuitable fit can leave gaps or interfere with safe laboratory work, while incorrect handling can move contaminants from equipment to skin, clothing, or nearby surfaces. Attention to fit, use, removal, and disposal therefore supports exposure reduction and limits cross-contamination.
PPE usage is the final personal barrier within a broader safety strategy, not a substitute for measures that control hazards at their source or organize work safely. Engineering and administrative controls help reduce the chance of exposure before equipment is needed. Combining these approaches provides layered protection for personnel working with cultures, specimens, or laboratory animals.
A sound process begins by identifying the relevant biological, chemical, and physical hazards, then selecting equipment suited to those exposures. The equipment must fit correctly during work and be handled carefully afterward. Removal and disposal deserve particular attention because contaminated PPE can spread material to skin, clothing, equipment, or surfaces if managed improperly.
These items protect through related but distinct barrier functions. Gloves help limit contact with contaminated materials and surfaces, laboratory coats protect clothing and the body, and eye protection or face shields address splashes. Masks contribute to protection when aerosols are a concern. Choosing among them depends on the exposure pathway and the work being performed.
PPE usage is particularly relevant during work with cultures, specimens, infectious materials, aerosols, contaminated surfaces, and laboratory animals. In these settings, consistent practices help reduce occupational exposure and accidental spread of biological agents. They also support reliable laboratory operations by limiting cross-contamination, provided that PPE is used alongside appropriate engineering and administrative controls.