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JoVE Journal
Biology

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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
 

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Article doi: 10.3791/53827
May 27th, 2016

Summary May 27th, 2016

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

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