Prolene Suture Fixation
Prolene Suture Fixation - Related Videos
05:22
Iris Fixation via External Pentagram Suturing
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2022
The present protocol describes how a 10-0 polypropylene suture crosses the anterior surface of the iris, forming a pentagram to prevent both iris and pupil from moving toward the cornea. This can be combined with subsequent keratoplasty to cure bullous keratopathy with extensive anterior synechia of the iris.
06:57
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Cited by 48 •
2015
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
04:51
Q Suture Technique: A Suturing Technique to Reduce Gap Formation and Increase Tensile Strength in Tendon Repair
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2025
In this video, we demonstrate suturing techniques — core suture and Q suture — to repair the foot flexor tendon in an ex vivo porcine model. Q sutures enhance the strength of the core suture in tendon repair and decrease gapping at the tendon repair site.
01:03
Fixation and Sectioning
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2023
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens. The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
FAQs
The fixation is produced by passing the thread through adjacent tissue and securing it with knots. Those knots help maintain tissue approximation and distribute tension across the repair rather than leaving support dependent on a single point of contact. This mechanical arrangement is important when the repaired area must remain positioned while biological healing or biomaterial integration proceeds.
The monofilament structure limits fluid wicking along the thread. As a result, the fixation material provides mechanical support without relying on a structure that readily carries fluid along its length. This property matters when the thread is used with living tissue, implants, or grafts, where material behavior can influence the local interaction between the repair and its surroundings.
Because the material is nonabsorbable, it remains available after placement rather than being removed through absorption. That persistence provides long-term mechanical support, which is valuable when tissue or an attached biomaterial needs continued stabilization. In biology research, the same feature allows investigators to examine healing, remodeling, and tissue interaction while the fixation remains present.
A basic workflow begins by positioning the tissue or biomaterial that requires support, then passing the suture through adjacent tissue. The thread is tightened and secured with knots so the structures remain approximated in a stable position. This sequence connects placement, tension distribution, and retention, making the fixation suitable for repairs or attachments that require durable support.
Applications include wound closure, tissue repair, and attachment of experimental implants or grafts. The method is especially relevant when the study or repair requires support that persists over time rather than disappearing soon after placement. In experimental settings, fixation therefore serves not only to hold material in position but also to support observation of how living tissue responds during healing and remodeling.
In a biological or biomedical model, the fixation can act as a stable interface between living tissue and an implant or graft. Researchers can then follow the relationship between persistent suture material and the surrounding tissue as healing and remodeling occur. This makes the method useful for investigating both mechanical stabilization and the interaction between a repair material and living tissue.