Recombinant Interferons
Recombinant Interferons - Related Videos
13:41
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Cited by 9 •
2012
Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.
03:11
Quantification of Bioactive Type-I Interferons using a Secreted Embryonic Alkaline Phosphatase Reporter Assay
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2025
This video demonstrates secreted embryonic alkaline phosphatase assay using HEK-Blue interferon-α/β reporter cells to quantify the type-I interferons (IFNs) released following stimulation of plasmacytoid dendritic cells by HIV-1 Infected CD4+ T cells.
02:17
An In Vitro Assay for Screening Interferons as Potent Inhibitors of Zika Virus Growth
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2025
The video demonstrates an in vitro assay for screening interferons with antiviral activity against the Zika virus, a human pathogen. Within the host cell, the Zika virus undergoes multiplication upon internalization, infecting neighboring cells and creating distinct zones or viral plaques. Type I interferon, acting as an antiviral agent, hinders virus multiplication, thereby preventing the formation of viral plaques in the host cell monolayer.
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
10:10
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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2016
This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.
FAQs
Binding to specific cell-surface receptors initiates the JAK-STAT signaling pathway, which carries the extracellular signal into the cell and activates interferon-stimulated genes. This connects a protein-receptor interaction with changes in gene expression, allowing investigators to examine how cells respond to antiviral signals, immune regulation, or infection-related conditions.
JAK-STAT signaling matters because it links receptor recognition to a defined cellular response rather than treating interferon activity as an isolated protein effect. Tracking this pathway helps researchers connect interferon exposure with activation of interferon-stimulated genes and then study consequences for antiviral defense, immune activity, cell growth, and host-pathogen interactions.
Bacteria and cultured mammalian cells are both identified as possible hosts for producing recombinant interferons. Using engineered host cells provides a controlled way to obtain the protein for research or therapeutic development. The available source does not specify that one host is universally superior, so host selection should be treated as an experimental or manufacturing decision rather than a fixed biological rule.
A basic workflow begins by inserting an interferon gene into a chosen host cell, such as a bacterium or cultured mammalian cell, so the host can produce the target protein. The resulting recombinant material can then support controlled investigations of receptor signaling, interferon-stimulated gene activation, or cellular responses to infection, depending on the experimental goal.
Therapeutic development uses recombinant interferons as candidates for addressing selected viral infections, cancers, and immune-related disorders. Their laboratory production makes it possible to obtain these signaling proteins in a controlled form for evaluating biological effects and potential medical uses. This application extends interferon research from cellular mechanisms toward interventions involving infection, abnormal cell growth, or immune regulation.
In biology research, recombinant interferons provide a controlled way to investigate host-pathogen interactions, immune regulation, and cellular responses to infection. Their activity can also help researchers examine cytokine signaling and gene expression through receptor engagement, JAK-STAT activation, and induction of interferon-stimulated genes. These outcomes connect molecular signaling events with broader cellular responses.