Sac Component Visualization
Sac Component Visualization - Related Videos
10:38
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
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Cited by 19 •
2008
This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.
11:53
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Cited by 6 •
2016
To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.
04:34
Dissection of the Endolymphatic Sac from Mice
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Cited by 5 •
2021
This study describes how to dissect the endolymphatic sac from the inner ear of the mouse at different ages. The result of a similar dissection is shown in an Slc26a4-null mouse model of enlargement of the endolymphatic sac. A transgenic mouse with a fluorescent reporter expressed in the endolymphatic sac is presented as a model to readily visualize the endolymphatic sac, and educational tool.
04:34
Surgical Treatment of an Endolymphatic Sac Tumor
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Cited by 1 •
2023
Here, we discuss clinical and radiological features of endolymphatic sac tumors and report the methodology and results of a surgical removal in a case in care at our otologic referral center.
08:08
Major Components of the Light Microscope
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2008
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.
FAQs
The method compares the spatial distribution of spindle assembly checkpoint proteins across successive mitotic stages. A component that appears at a particular location, remains there, or disappears as chromosomes attach to the spindle provides visual evidence of checkpoint assembly, persistence, or dissociation. This timing helps investigators relate protein behavior to chromosome segregation accuracy.
Structural markers provide the positional framework needed to interpret checkpoint-protein signals. Kinetochore labeling identifies chromosome-associated regions, whereas microtubule labeling shows the spindle structures involved in chromosome attachment. Comparing these signals indicates where checkpoint components are located relative to chromosomes and spindle fibers, helping distinguish stage-specific localization from general cellular distribution.
Differences in fluorescence patterns between mitotic stages can show when checkpoint proteins assemble, persist, or dissociate during cell division. Examining these transitions alongside chromosome and spindle markers connects protein localization with changes in spindle attachment. The resulting comparisons support analysis of how mitotic regulation is coordinated over the course of chromosome segregation.
A typical workflow fixes cells, labels checkpoint components with antibodies, and adds structural markers for kinetochores or microtubules. The prepared cells are then examined by fluorescence microscopy, with images compared across mitotic stages. This sequence preserves cellular organization for imaging and enables researchers to evaluate the relative locations of checkpoint proteins and division structures.
Interpretation focuses on where fluorescence signals occur and how their distribution changes across mitotic stages. Investigators compare checkpoint-protein labeling with kinetochore and microtubule signals to determine whether components are associated with chromosome regions or spindle structures. These comparisons can reveal patterns of assembly, persistence, and dissociation rather than merely showing that a protein is present.
The approach is useful when researchers need to examine mitotic regulation and chromosome segregation accuracy at the cellular level. It also supports studies of chromosome instability, developmental biology, and diseases linked to faulty cell-cycle control. By showing checkpoint-protein behavior in relation to division structures, the method connects molecular localization with broader outcomes of cell-cycle regulation.