Seconday Metabolites

Secondary metabolites are specialized organic compounds produced by plants, fungi, and microorganisms that are not directly required for basic growth but can influence survival, ecological interactions, and human health. They arise from regulated biosynthetic pathways that modify primary metabolic intermediates into chemically diverse molecules, including alkaloids, terpenoids, phenolics, and antibiotics. These compounds help organisms deter predators, inhibit competitors, attract pollinators, or tolerate environmental stress. In biology and biotechnology, studying secondary metabolites supports drug discovery, antimicrobial development, agriculture, and the production of natural products with pharmaceutical, nutritional, and industrial value.

Seconday Metabolites - Related Videos

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JoVE Journal - Biology
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Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

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2025

This protocol describes a high-throughput approach for evaluating plant ion leakage, peroxidase activity, and callose production in the same sample.

Research

JoVE Journal - Environment

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

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Cited by 6 •

2015

This bioassay employs a model predatory fish to assess the presence of feeding-deterrent metabolites from organic extracts of the tissues of marine organisms at natural concentrations using a nutritionally comparable food matrix.

13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

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2024

The developed method of 13C6-Glucose labeling combined with liquid chromatography high-resolution mass spectrometry is versatile and lays the foundation for future studies on the primary organs and pathways involved in the synthesis of secondary metabolites in medicinal plants, as well as the comprehensive utilization of these secondary metabolites.

Detection of Human Fecal Metabolites Using a Bacterial Biosensor

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2026

Begin with a bacterial biosensor culture suspended in a medium.These engineered bacteria produce a transcription factor that, upon binding to a specific fecal metabolite, activates a promoter associated with a fluorescent reporter gene.To this culture, add a filtered human fecal sample containing metabolites, such as lactate, that are involved in host-microbiome interactions.Dilute this mixture with a fresh medium to maintain an optimal reaction volume.Incubate without shaking to mimic the...

Research

JoVE Journal - Biology
Free Sample

Purification of Transcripts and Metabolites from Drosophila Heads

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Cited by 12 •

2013

We describe here the procedures for the extraction and purification of mRNA and metabolites from Drosophila heads. We are applying these techniques to better understand the cellular perturbations underlying neuronal degeneration. These methodologies can be easily scaled and adapted for other "omic" projects.

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