Purification of Transcripts and Metabolites from <em>Drosophila</em> Heads

Kurt Jensen; Jonatan Sanchez-Garcia; Caroline Williams; Swati Khare; Krishanu Mathur; Rita M. Graze; Daniel A. Hahn; Lauren M. McIntyre; Diego E. Rincon-Limas; Pedro Fernandez-Funez

doi:10.3791/50245

Journal

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Biology

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Purification of Transcripts and Metabolites from Drosophila Heads

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Purification of Transcripts and Metabolites from Drosophila Heads

Purification of Transcripts and Metabolites from Drosophila Heads

21,688 Views

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12:49 min

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March 15, 2013

DOI:

10.3791/50245-v

DOI:

10.3791/50245-v

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12:49 min

March 15, 2013

•

4 Views

Kurt Jensen¹, Jonatan Sanchez-Garcia¹, Caroline Williams², Swati Khare¹, Krishanu Mathur¹, Rita M. Graze³, Daniel A. Hahn², Lauren M. McIntyre³, Diego E. Rincon-Limas^1,4, Pedro Fernandez-Funez^1,4

¹Department of Neurology, McKnight Brain Institute,University of Florida , ²Department of Entomology and Nematology,University of Florida , ³Genetics Institute, Department of Molecular Genetics and Microbiology,University of Florida , ⁴McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration,University of Florida

Summary

We describe here the procedures for the extraction and purification of mRNA and metabolites from Drosophila heads. We are applying these techniques to better understand the cellular perturbations underlying neuronal degeneration. These methodologies can be easily scaled and adapted for other "omic" projects.