Skin Specimen
Skin Specimen - Related Videos
08:12
Brain Banking: Making the Most of your Research Specimens
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Cited by 21 •
2009
Brain banking and systematic sampling of biological material provides the basis for unbiased stereology and maximizes the potential data obtained from each specimen.
06:53
Cell Population Analyses During Skin Carcinogenesis
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Cited by 6 •
2013
Carcinogenesis is a process involving interactions between cancer cells and the cancer microenvironment. To dissect the molecular events, one needs to isolate different cell populations at different stages during cancer development. Using a mouse model for basal cell carcinoma, we describe a protocol for cell analyses during carcinogenesis.
09:30
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Cited by 44 •
2016
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
04:23
Frozen Skin Tissue Block Preparation: A Protocol to Preserve Cutaneous Melanoma Samples from Murine Dorsal Skin
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2023
This video describes the detailed protocol for isolating melanoma-containing murine dorsal skin, followed by cryo-embedding to prepare a frozen skin tissue block. The cryo-embedded tissue block can be sectioned and microscopically examined to study melanoma progression.
05:44
Skin Biopsy for Diagnosing Discoid Lupus Erythematosus
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Cited by 2 •
2025
Skin lesions are common and important features in many rheumatic diseases, and biopsy with pathological analysis plays a critical role in diagnosis. This article provides a detailed overview of the standardized skin biopsy procedure and, using discoid lupus erythematosus as an example, highlights the essential role of dermatopathology in diagnosing rheumatic diseases.
FAQs
These preparation choices determine which features remain available for examination. Biopsy provides the collected tissue, while fixation or freezing preserves it for later analysis. Preservation supports sectioning, staining, microscopy, or molecular testing, allowing investigators to examine tissue organization or cellular activity without treating every specimen as suitable for the same analytical purpose.
Examining these components together preserves the tissue context needed to relate structure to function. Differences among the epidermis, dermis, and associated structures can help investigators study organization, barrier function, wound healing, inflammation, infection, or disease-associated changes. This layered context connects cellular observations with broader biological processes occurring within skin tissue.
Cellular activity can provide evidence about how skin tissue is maintained, responds to injury, or changes during inflammation, infection, and disease. Depending on preparation and analysis, investigators may use staining, microscopy, or molecular methods to connect visible tissue changes with biological activity. The resulting observations help distinguish structural organization from processes affecting tissue condition.
A typical workflow begins with collecting the sample through biopsy, followed by fixation or freezing to preserve it. The material can then be sectioned and stained for microscopic examination, or directed toward molecular analysis. Selecting the preparation and analysis sequence helps ensure that the specimen retains the structural or cellular information required by the study.
Microscopy is useful when the study requires direct examination of tissue organization or stained structures. Molecular analysis is appropriate when investigators need information about cellular activity at the molecular level. Because the overview identifies both approaches as complementary options, researchers can select one or combine them according to whether the main question concerns architecture, activity, or both.
Skin specimens provide biological material for evaluating cosmetics, therapeutics, and drug delivery systems, as well as tissue-engineered substitutes. Analyses can connect treatment or material exposure with changes in tissue organization and cellular activity. In this way, specimens support comparisons between biological responses and the intended performance of products or replacement tissues.