Swarmer Cell Isolation
Swarmer Cell Isolation - Related Videos
03:44
Preparation of Vibrio parahaemolyticus Swarmer Cells for Imaging
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2025
Source: Heering, J., et al., Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells. J. Vis. Exp. (2017).This video demonstrates the preparation of swarmer cells from a Vibrio parahaemolyticus colony for fluorescence imaging. The bacteria are genetically engineered to express a fluorophore-tagged protein that localizes unipolarly in swimmer cells and bipolarly in elongated swarmer cells, allowing for their visual differentiation. An...
03:55
Induction of Differentiation of Vibrio parahaemolyticus into Swarmer Cells
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2025
Source: Heering, J., et al. Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells. J. Vis. Exp. (2017)This video demonstrates the induction of swarmer cell differentiation in Vibrio parahaemolyticus using a specially prepared agar medium. By manipulating environmental cues such as iron depletion, calcium supplementation, and surface contact, the bacteria are stimulated to transition from their swimmer form into elongated swarmer cells...
08:38
Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
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Cited by 17 •
2017
This protocol enables single cell microscopy of the differentially distinct Vibrio parahaemolyticus swimmer and swarmer cells. The method produces a population of swarmer cells easily available for single cell analysis and covers preparation of cell cultures, induction of swarmer differentiation, sample preparation, and image analysis.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
FAQs
Surface contact and nutrient conditions act as environmental signals that can shift vegetative cells toward a differentiated, motile state. During this transition, cells elongate and increase flagellation, changes that support collective migration across solid media. Because these cues influence whether swarming develops, researchers can examine how environmental conditions regulate bacterial behavior and population organization.
Elongation and increased flagellation are cellular changes associated with the swarmer state. They help distinguish differentiated, motile cells from non-swarming counterparts and provide observable features for studying the biology of surface-associated movement. Examining these traits can connect cell morphology with coordinated migration, gene regulation, and the organization of bacterial populations.
The advancing swarm edge represents a growth zone where coordinated movement is actively extending across the surface. Collecting cells from this region can therefore enrich analysis of the differentiated population associated with migration rather than the surrounding, non-swarming cells. This location is useful for investigating how individual cellular changes contribute to organized movement and surface colonization.
A typical approach begins by allowing vegetative bacterial cells to encounter solid-media conditions that promote surface-associated migration. Once a swarm develops, cells are collected from the advancing edge or another relevant growth zone. The recovered population can then be examined for motility, differentiation, gene regulation, or other traits linked to the swarming state.
The comparison reveals how bacterial cells change when they transition from a vegetative, non-swarming state to a differentiated motile state. Researchers can examine differences in elongation, flagellation, movement, and regulatory behavior. These contrasts help identify features associated with coordinated migration and clarify how environmental responses produce distinct cellular states within a population.
This method supports studies of bacterial motility, cellular differentiation, gene regulation, and population organization. It also provides a way to examine how coordinated movement may contribute to colonization, persistence, and infection-related traits. By focusing on cells recovered from migration-associated zones, researchers can connect surface behavior with broader biological consequences.