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JoVE Journal
Immunology and Infection

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Isolation of Functional Cardiac Immune Cells

Isolation of Functional Cardiac Immune Cells

Article DOI: 10.3791/3020
December 5th, 2011


Summary December 5th, 2011

This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.

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