Synthetic Rna Introduction

Synthetic RNA introduction is the delivery of laboratory-produced RNA molecules into cells to direct, regulate, or measure biological activity. Researchers design a specific RNA sequence, produce it by chemical synthesis or in vitro transcription, and introduce it through methods such as lipid-based carriers or electroporation; once inside, the RNA can be translated into protein, guide gene regulation, or trigger RNA interference. This approach enables transient gene expression without altering genomic DNA and supports studies of cell function, gene regulation, vaccine development, and therapeutic design. Its controlled timing and sequence specificity also make it valuable for investigating molecular mechanisms and testing engineered biological systems.

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Education

JoVE Science Education - Engineering

Synthetic Biology

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2023

This video presents synthetic biology and its role in bioengineering. Synthetic biology refers to the methods used to genetically modify organisms in order to make them capable of producing large quantities of a product. This product could be a protein that the cell already makes or a new protein that has been encoded in a newly-inserted DNA sequence. Here, we discuss how an organism's genetic material is modified using transformation or transfection. Then, the process is shown in the...

Research

JoVE EoE - Viral Growth and Techniques

Rescue of Infectious Virions from Cells Transfected with Bacterial Artificial Chromosome-Derived Synthetic RNA

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2026

Source: Yun, S. et al. Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses. J. Vis. Exp. (2015)This video demonstrates the recovery of infectious Japanese encephalitis virus from synthetic RNA transcribed from a bacterial artificial chromosome clone. It shows how cells are electroporated with the synthetic RNA, allowing the viral genome to direct the production of new virions. The resulting plaques formed in infected cell monolayers confirm...

Research

JoVE Journal - Biology
Free Sample

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

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Cited by 4 •

2010

This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.

Production of Synthetic Nuclear Melt Glass

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Cited by 1 •

2016

A protocol for the production of synthetic nuclear melt glass, similar to trinitite, is presented.

Introduction to the Spectrophotometer

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2023

The spectrophotometer is a routinely used instrument in scientific research. Spectrophotometry is the quantitative measurement of how much a chemical substance absorbs light by passing a beam of light through the sample using a spectrophotometer. In this video, basic concepts in spectrophotometry, including transmittance, absorbance and the Beer-Lambert Law are reviewed in addition to the components of the spectrophotometer. These concepts provide a foundation for how to determine the...

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