Temporal Fluctuations

Temporal fluctuations are changes in the magnitude or timing of biological variables over time, and they matter because organisms and ecosystems respond to conditions that are rarely constant. In cells, fluctuations can arise from stochastic molecular events, rhythmic regulation, or feedback within gene-expression and signaling networks, while environmental variables such as temperature, nutrients, and population density can vary across broader timescales. Studying these dynamics helps researchers distinguish noise from regulated behavior, identify biological rhythms, and understand how organisms maintain function under changing conditions. Temporal fluctuation analysis also supports research on development, disease progression, ecological stability, and responses to environmental stress.

Temporal Fluctuations - Related Videos

Education

JoVE Business - Macroeconomics

Seasonal Fluctuations

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2026

Seasonal fluctuations refer to regular and predictable changes in economic activity that happen at specific times each year. These variations arise from recurring influences such as weather conditions, holidays, and institutional schedules. Unlike business cycles, which are irregular, seasonal fluctuations are predictable and happen every year.Weather patterns can affect the economy. Certain industries, such as agriculture and construction, tend to experience higher or lower activity during...

Research

JoVE EoE - Biomolecular Interaction Detection Techniques

Fluorescence Fluctuation Spectroscopy to Study Protein Homo-Oligomerization

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2025

This video demonstrates the technique of fluorescence fluctuation spectroscopy (FFS) to study protein homo-oligomerization. Fluorescently-labeled proteins in a sample are dimerized using an agent when studying protein oligomerization using FFS. Using a confocal microscope, as the proteins move in and out of the small observation volume, the analysis of the fluctuations in the brightness of the fluorescent molecules is performed to determine the oligomeric state of the proteins.

Fluorescence Fluctuation Spectroscopy to Study Protein Interaction at Cell Contacts

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2025

This video demonstrates the use of fluorescence fluctuation spectroscopy to detect the interaction among cell surface proteins at cell-cell contacts. By expressing the transmembrane adhesion receptor of interest labeled with a fluorescent protein and mixing two different cell populations harboring two spectrally separated fluorescent labels, the trans-interaction between the receptors of two neighboring cells with different-colored fluorescence is assessed via cross-correlation in the...

Research

JoVE Journal - Bioengineering
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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

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Cited by 5 •

2015

Inter-cellular communication is critical for controlling various physiological activities within and outside the cell. This paper describes a protocol for measuring the spatio-temporal nature of single cell secretions. To achieve this, a multidisciplinary approach is used which integrates label-free nanoplasmonic sensing with live cell imaging.

Research

JoVE Journal - Genetics
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Measuring Microbial Mutation Rates with the Fluctuation Assay

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Cited by 35 •

2019

Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.

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