Tissue Localization
Tissue Localization - Related Videos
02:37
CT-guided Preoperative Localization of Pulmonary Nodules Using a Glucose Test and Tissue Adhesive
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2026
This CT-guided protocol localizes ground-glass nodules preoperatively using tissue adhesive and a glucose test, which verifies needle placement and prevents airway injection-enhancing safety and distinguishing it from prior localization techniques.
09:29
Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens
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Cited by 19 •
2016
We describe the use of digital image correlation to characterize the local surface strain field on vascular tissue samples subjected to uniaxial tensile testing. These measurements facilitate precise quantification of the sample mechanical response and the generation of constitutive stress-strain relations.
01:24
Tissue-Drug Binding: Localization of Drugs and its Significance
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2025
Body tissues, comprising approximately 40% of the body weight, are crucial in drug distribution and localization. These tissues can serve as drug storage sites, competing with plasma binding sites for drug molecules. Drugs can bind to different tissue components, enhancing their distribution and localization. The factors influencing drug localization in tissues include the drug's lipophilicity, structural characteristics, tissue perfusion rate, and pH differences. These factors determine the...
02:42
Imaging Bacterial Localization and Host Interaction in Human Lung Tissue
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2026
Source: Mills, B., et al., Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy. J. Vis. Exp. (2017)This video demonstrates real-time imaging of fluorescently labeled Staphylococcus aureus in suspension and lung tissue using fibered confocal fluorescence microscopy.
07:05
Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
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Cited by 2 •
2018
A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.
FAQs
Preserving tissue architecture keeps cells, molecular targets, and anatomical regions in their original spatial relationships. This allows a detected protein or nucleic acid signal to be interpreted within a defined cellular or tissue context rather than as an isolated measurement. The resulting spatial pattern can reveal organization, local signaling, and relationships between neighboring structures.
Immunohistochemistry detects target proteins, whereas in situ hybridization detects nucleic acids within preserved tissue regions. Their signals therefore address different biological questions, such as where a protein is situated versus where related gene expression occurs. Comparing these patterns can help connect molecular identity with tissue organization and functional interpretation.
Fluorescent labeling marks selected biological targets so microscopy can visualize their distribution within tissue. The important information is not only whether a signal is present, but also which cells or anatomical regions contain it. This spatial readout helps researchers examine cell organization, gene expression patterns, and localized changes associated with disease.
A typical workflow preserves the tissue’s architecture, applies a detection approach suited to the target, and uses microscopy to examine the resulting signal. Researchers then relate the detected protein or nucleic acid to defined cells or anatomical regions. This sequence converts molecular detection into spatial information that can be interpreted biologically.
The choice depends largely on the type of target and the spatial information required. Immunohistochemistry is suited to protein detection, while in situ hybridization addresses nucleic acids. Fluorescent labeling and microscopy provide ways to visualize signals in tissue. Selecting the appropriate combination helps align the measurement with questions about expression, organization, or disease-related changes.
Tissue localization can show where gene expression, proteins, cells, or structures occur within an organized tissue. These patterns support studies of development, pathology, neuroscience, and biomedical biology by connecting molecular identity with position. In turn, researchers can investigate how local interactions contribute to tissue structure, function, and disease-related alterations.