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Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
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Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

Article DOI: 10.3791/58232-v • 07:05 min • October 4th, 2018
October 4th, 2018 •
1, 1, 2, 3, 1,2, 4, 1,2, 1,2
1Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, 2School of Veterinary Medicine, National Taiwan University, 3Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 4Graduate Institute of Veterinary Pathobiology, National Chung Hsing University

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Summary
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A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.

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Autometallography Localize Semi-quantify Silver Cetacean Tissues Environmental Toxicology Heavy Metals AMG Methodology Tissues Dolphins Whales Wen-Ta Li Shun Theng Clinician Liver Kidney Formalin-fixed Tissues Stainless Steel Microtome Blades Sections Labeled Cassettes Tissue Processor Graded Ethanol Xylene Immersions Paraffin Immersions Histology Molds
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