Tissue Specific Imaging
Tissue Specific Imaging - Related Videos
07:06
Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
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Cited by 6 •
2014
Described here is a protocol for semi-automated imaging of tissue-specific fluorescence in zebrafish embryos.
10:28
High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
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2025
Spectral Iterative Bleaching Extends Multiplexity (IBEX) builds upon the base IBEX technique by adding heparin blocking to minimize nonspecific binding and leveraging spectral detection with computational unmixing to suppress autofluorescence. This approach accelerates image acquisition while reducing sources of background, enabling robust multi-round, high-parameter spatial proteomic analyses.
08:34
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Cited by 59 •
2016
We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.
03:33
Tissue Immunostaining for Imaging Mass Cytometry
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2026
Source:Jake Griner1, Arwen Wedgeworth2, Brenton Puckett2, Kacey Idouchi1, Slivia Guglietta2, Carsten Krieg11Department of Pathology and Laboratory Medicine, Medical University of South Carolina.2Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina.The video describes the preparation of a tissue sample for imaging mass cytometry. The tissue is first processed to unmask the antigen epitopes, permeabilize the cells, and block non-specific antibody binding...
08:12
A Web-Based Workflow for Selecting Gene- and Tissue-Specific Enhancers
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2025
We present a coding-free workflow for biologists to identify tissue-specific gene enhancers using only browser-based tools. Our protocol leverages public H3K4me1/H3K27ac histone marks and Hi-C data, enabling researchers without programming expertise to access, analyse, and identify potential regulatory elements associated with their genes of interest.
FAQs
Selectivity arises when an imaging signal accumulates in or binds to characteristic cellular components. Fluorescent labels and stains make selected structures visible, while targeted contrast agents provide localized contrast within the specimen or organism. Because surrounding regions receive a different or weaker signal, researchers can distinguish tissue-specific features and relate their distribution to cellular organization or molecular activity.
The approach combines visual information from imaging modalities with signals linked to particular cellular components. This allows researchers to examine where tissues and cells are arranged while also observing associated molecular features. The resulting view helps connect microscopic mechanisms, such as molecular activity or cell distribution, with larger patterns of tissue organization and whole-tissue outcomes.
Tissue-selective signals can reveal differences in tissue architecture, cell distribution, and molecular activity across a specimen or organism. These patterns may change during development, injury, or disease. Comparing the selected regions and their signals therefore helps researchers characterize biological changes rather than viewing the specimen as a uniform structure.
A study first identifies the tissue, structure, or molecular feature that must be distinguished, then selects an imaging modality and a compatible signal, such as a fluorescent label, stain, or targeted contrast agent. Imaging reveals the selected regions within the surrounding specimen. Researchers can then interpret tissue architecture, cell distribution, or molecular activity in biological context.
Researchers may use it for anatomical mapping, functional studies, disease characterization, or evaluation of experimental treatments. The most appropriate application depends on whether the study needs to locate tissues, examine biological activity, describe disease-associated changes, or assess treatment-related outcomes. In each case, selective visualization links a specific region or feature to the broader biological question.
By distinguishing selected tissues and molecular features from surrounding regions, the method can show how architecture, cell distribution, or molecular activity changes under different biological conditions. In developmental studies, it supports mapping changes over time; in injury or disease studies, it helps characterize altered tissue patterns. These observations can also support evaluation of experimental treatments.