Tissue Targeting
Tissue Targeting - Related Videos
02:42
Immunohistochemistry of Mouse Brain Tissue Sections for Targeting Dopamine Neurons
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2025
Source: Kummari, E., et. al., Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area., J. Vis. Exp. (2015)In this video, we demonstrate the immunohistochemistry protocol for dopamine neurons in brain tissue sections for subsequent laser capture microdissection.
04:39
Quantitative Dot Blot to Estimate Target Proteins in a Tissue Lysate
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2025
This video demonstrates a quantitative dot blot, or QDB, technique for the absolute quantification of proteins of interest in a tissue sample. The proteins in the sample are immobilized on the nitrocellulose membrane inside the wells of a QDB plate. Utilizing target protein-specific antibodies labeled with a peroxidase enzyme, a chemiluminescent substrate is oxidized to produce light, which is measured to determine the protein concentration of the sample.
18:57
Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation
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Cited by 12 •
2009
Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
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2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
06:37
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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Cited by 7 •
2021
This article outlines a detailed protocol for using the RNA-targeting Cas13D enzyme (RfxCas13D) in flies.
FAQs
Tissue targeting can exploit differences in anatomy, blood flow, vascular permeability, surface receptors, and extracellular-matrix composition. These features determine where an agent can travel, cross vessel walls, bind, or remain localized. Selecting the most informative tissue property helps align the delivery strategy with the biological barrier or recognition signal that distinguishes the intended site from other tissues.
Targeting ligands, engineered carriers, and tissue-specific promoters act at different stages of localization. Ligands can support recognition through surface receptors, carriers can help direct cargo, and promoters can restrict gene activity to selected tissues. Local tissue conditions may then influence uptake or release, so delivery to a site does not necessarily produce uniform activity throughout it.
Heterogeneous distribution and rapid clearance are major reasons that apparent targeting may be incomplete. Portions of a tissue may differ in access, permeability, receptor availability, or extracellular matrix, while clearance can reduce the amount that remains available. Evaluating both localization and persistence is therefore important when judging efficacy, imaging quality, or off-target exposure.
A tissue-targeting workflow begins by identifying distinguishing tissue properties and selecting a compatible ligand, carrier, promoter, or combination. The chosen material is then assessed for localization, uptake, and, where relevant, release under local conditions. Comparing the intended tissue with other sites reveals whether the strategy improves distribution and limits unintended exposure.
In biology, this approach supports disease modeling, molecular imaging, gene delivery, drug delivery, and regenerative research. The desired readout depends on the application: imaging focuses on localization, delivery studies examine cargo placement and activity, and regenerative work may use tissue-specific localization to investigate biological function. These uses connect spatial control with experimental interpretation.
It can clarify tissue function by linking a biological effect or imaging signal to a selected anatomical site. In therapeutic studies, improved localization may increase efficacy while reducing off-target effects. In research models, however, uneven distribution or rapid clearance must be considered before attributing an outcome solely to tissue-specific activity.