Topographic Imaging

Topographic imaging is a set of imaging approaches that maps the three-dimensional shape, height, and surface relief of a specimen, helping biologists relate structure to function. The technique records spatially varying signals, such as probe-surface interactions or optical depth information, and converts them into a height map or surface model that reveals features not visible in a conventional two-dimensional image. In biology, topographic imaging supports analysis of cell morphology, tissue organization, membrane features, and biomaterial surfaces, while enabling quantitative measurements of surface architecture and changes caused by development, disease, or experimental treatment.

Topographic Imaging - Related Videos

Education

JoVE Science Education - Environmental Sciences

Using Topographic Maps to Generate Topographic Profiles

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2023

Source: Laboratory of Alan Lester - University of Colorado Boulder Topographic maps are "plan-view" representations of Earth's three-dimensional surface. They are a standard type of map-view that provides an overhead, or aerial, perspective. Among the defining features of a topographic map are the contour lines that indicate locations of constant elevation. The elevation interval between the contour lines is dependent on the level of detail provided by the map and the kind of topography present.

Topographic Surveying and Contours

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2025

Topographic surveying is critical for documenting the Earth's surface, focusing on capturing elevations, slopes, and natural and man-made features. It is essential in construction planning, water resource management, and land-use analysis. The primary outcome of such surveys is a topographic map, which uses contour lines to visually represent the shape and slope of the terrain, providing valuable insights into the landscape's characteristics.Contour lines are fundamental to understanding the...

Plotting of Topographic Maps

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2025

Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...

Research

JoVE Journal - Behavior

Topographical Estimation of Visual Population Receptive Fields by fMRI

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Cited by 3 •

2015

It is important to obtain unbiased estimates of visual population receptive fields (pRFs) by functional magnetic resonance imaging. We use mild regularization constraints to estimate pRF topography without a-priori assumptions about pRF shape, allowing us to choose specific pRF models post-hoc. This is particularly advantageous in subjects with visual-pathway lesions.

Research

JoVE Journal - Environment
Free Sample

Use of Principal Components for Scaling Up Topographic Models to Map Soil Redistribution and Soil Organic Carbon

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Cited by 6 •

2018

Landscape processes are critical components of soil formation and play important roles in determining soil properties and spatial structure in landscapes. We propose a new approach using stepwise principal component regression to predict soil redistribution and soil organic carbon across various spatial scales.

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