Undifferentiated Rhizobia

Undifferentiated rhizobia are nitrogen-fixing bacteria that establish symbiotic associations with legume roots without undergoing terminal differentiation into specialized bacteroids. During symbiosis, the bacteria enter root cells within infection threads and membrane-bound nodules, where plant-derived carbon supports bacterial respiration and the nitrogenase enzyme converts atmospheric nitrogen into ammonia for plant use. Because undifferentiated rhizobia retain a more typical bacterial form and may preserve reproductive capacity, they provide a useful contrast to terminally differentiated rhizobia in studies of nodule development, cellular specialization, and host–microbe signaling. This biology also informs research on biological nitrogen fixation and sustainable strategies for improving soil fertility and crop productivity.

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Education

JoVE Science Education - Environmental Sciences

Bacterial Growth Curve Analysis and its Environmental Applications

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Bacteria are among the most abundant life forms on Earth. They are found in every ecosystem and are vital for everyday life. For example, bacteria affect what people eat, drink, and breathe, and there are actually more bacterial cells within a person’s body than mammalian cells. Because of the importance of bacteria, it is preferable to study particular species of bacteria...

Research

JoVE Journal - Biology
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Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

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Cited by 170 •

2012

The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgfβ1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.

A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field

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Cited by 26 •

2012

In this protocol we demonstrate how to construct custom chambers that permit the application of a direct current electric field to enable time-lapse imaging of adult brain derived neural precursor cell translocation during galvanotaxis.

Research

JoVE Journal - Biology
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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

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Cited by 38 •

2011

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

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Cited by 19 •

2016

Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.

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