We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.
Parsons, X. H., Teng, Y. D., Parsons, J. F., Snyder, E. Y., Smotrich, D. B., Moore, D. A. Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction. J. Vis. Exp. (57), e3274, doi:10.3791/3274 (2011).