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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
JoVE Journal
Biology
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JoVE Journal Biology
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

DOI:
10.3791/3274-v

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10:46 min

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November 03, 2011

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, , , , ,
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery,Harvard Medical School, 4Division of SCI Research,VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Chapters

  • 00:05Title
  • 01:22Media Preparation and Plate Coating
  • 02:30Passing and Seeding Undifferentiated Human ES Cells
  • 04:27Cardiac Induction in Adhesive Culture with Nicotinamide
  • 05:37Differentiation in Suspension Culture and Maturation to Beating Cardiomyocyte Phenotype after Attachment
  • 08:05Direct Cardiomyocyte Differentiation of Feeder- and Serum-Free Pluripotent Humand ES Cells with Nicotinamide Induction
  • 10:23Conclusion

Summary

Automatic Translation

Automatic Translation

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.

Tags

Human Cardiac PrecursorsCardiomyocytesPluripotent Human Embryonic Stem CellsSmall Molecule InductionRegenerating Human MyocardiumCell-based TransplantationStem/progenitor CellsBone MarrowCord BloodContractile Heart Muscle CellsAdult Stem Cell TherapyGenetically Stable Human Embryonic Stem CellsUnlimited Expansion AbilityUnrestricted PlasticityLarge Supplies Of Human Somatic CellsCardiovascular DiseaseDonor OrgansHESC-based Therapies

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