Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons; Yang D. Teng; James F. Parsons; Evan Y. Snyder; David B. Smotrich; Dennis A. Moore

doi:10.3791/3274

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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

JoVE Journal
Biology

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JoVE Journal Biology

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

19,345 Views

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10:46 min

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November 03, 2011

DOI:

10.3791/3274-v

DOI:

10.3791/3274-v

•

10:46 min

November 03, 2011

•

1 Views

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery,Harvard Medical School, ⁴Division of SCI Research,VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

Summary

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.