Variable Volume Fed-batch
Variable Volume Fed-batch - Related Videos
01:23
Fed-Batch Culture
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2026
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
10:27
VFD-fed AC Induction Machine
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2023
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. Variable frequency drives (VFDs) are a type of adjustable speed drive, which are becoming standard equipment to power most AC induction motors. VFDs are common in industrial and automation applications and typically provide robust control of the motor in speed, torque, or position modes. The VFDs tested and simulated in this experiment focus on speed and open-loop control with constant voltage to...
08:07
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
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Cited by 5 •
2009
Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.
01:14
Batch vs Continuous Culture
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2026
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
FAQs
Feed-rate adjustment regulates how quickly nutrients enter the culture, helping control substrate availability during growth. Supplying nutrients at a suitable rate can reduce the likelihood of excess substrate and inhibitory byproduct formation while continuing to support cellular or microbial activity. This balance helps maintain productive conditions and may extend the culture period for biomass or recombinant protein production.
As feed accumulates, the working volume rises, which changes the conditions for oxygen transfer within the bioreactor. The addition rate therefore must be considered alongside the culture’s oxygen-transfer needs, not only its nutrient demand. Managing these linked variables helps sustain growth and productivity as the culture becomes larger during the process.
Batch culture does not provide the same volume increase associated with ongoing feed addition. In Variable Volume Fed-batch, nutrient delivery and working-volume changes can be used to maintain substrate availability over a longer productive period. This strategy may improve biomass or recombinant protein yields compared with batch culture when the process is appropriately controlled.
The process begins with cells or microorganisms growing in a bioreactor, followed by addition of a nutrient-rich feed. As the feed accumulates, the working volume increases, and the addition rate is adjusted in response to substrate availability, byproduct formation, oxygen-transfer conditions, and growth needs. Production then continues under these changing culture conditions.
Researchers may choose this approach when they want to support extended growth or production rather than rely solely on an initial nutrient supply. It is relevant when controlling substrate availability, limiting inhibitory byproduct formation, and sustaining oxygen transfer are important. These goals make the strategy useful for biomass, recombinant protein, enzyme, vaccine, and other biologically derived product production.
Applications span microbial fermentation and mammalian cell culture, so the strategy can support different biological production systems. In these settings, it is used for biopharmaceuticals, enzymes, vaccines, recombinant proteins, and other biologically derived products. Its value lies in coordinating nutrient delivery with growth and production requirements as the culture volume increases.