Fixed Volume Fed-batch
Fixed Volume Fed-batch - Related Videos
01:23
Fed-Batch Culture
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2026
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
16:31
Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock
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Cited by 24 •
2011
The Hemorrhagic Shock model has been a reliable and reproducible resource facilitating the identification and understanding of signaling cascades associated with inflammation and end-organ damage after trauma. This article provides a step-by-step description of surgical and mechanical aspects associated with the Hemorrhagic Shock experimental procedure in mice.
05:35
Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume
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2023
This video describes a simple and reliable method of introducing liquid into the trachea for lung instillation, fixation, and lung volume measurement.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
01:07
Conservation of Mass in Fixed, Nondeforming Control Volume
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2025
The principle of conservation of mass is fundamental in fluid dynamics and is crucial for analyzing flow within fixed control volumes, such as pipes or ducts. This principle states that the total mass within a control volume remains constant unless altered by the inflow or outflow of mass through the control surfaces. This results in a vital relationship for steady, incompressible flow where the mass entering a system equals the mass leaving it. In the case of a sewer pipe, which can be modeled...
FAQs
The matched inflow and outflow create a volume balance that allows nutrient delivery without progressive expansion of the culture volume. Operators can therefore adjust the supply of fresh medium while maintaining a stable working volume. This balance is important because it links feeding decisions with the culture’s dilution conditions, substrate availability, and removal of medium containing metabolic byproducts.
Controlling these factors helps keep the culture environment suitable for continued cell or microorganism growth. Fresh medium addresses the risk of nutrient depletion, while culture removal limits the accumulation of inhibitory byproducts. Managing dilution also supports more predictable exposure to incoming nutrients, which can influence biomass formation, product formation, and overall process performance.
The defining operational difference is whether feeding causes the culture volume to rise. In a fixed-volume process, an equal amount of culture leaves as fresh medium enters, so the reactor volume remains stable. This gives operators a way to regulate nutrient and waste conditions without the volume changes associated with processes that only add medium during cultivation.
Operation begins by supplying fresh medium to the bioreactor at a controlled rate. At the same time, an equal volume of culture is removed to preserve the working volume. The feed and removal rates are coordinated as the culture grows, allowing operators to manage substrate concentration, dilution, and metabolic waste while maintaining the intended cultivation conditions.
Researchers may select this approach when a culture requires stable environmental conditions and predictable process performance during growth or production. Supported applications include microbial fermentation, mammalian cell culture, recombinant protein production, and other biological systems in which nutrient supply, byproduct removal, and reactor volume need coordinated control.
By reducing the likelihood of nutrient depletion and inhibitory byproduct accumulation, the strategy can support continued biomass formation or product formation. Its controlled volume and feeding conditions also help make process behavior more predictable. In biology, these features are relevant when cultivating microorganisms or cells for fermentation, recombinant proteins, or other products requiring regulated culture conditions.