Vascularization
Vascularization - Related Videos
08:18
Engineered Vascularized Muscle Flap
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Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Cited by 37 •
2017
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
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Cited by 7 •
2014
The rodent retina has long been recognized as an accessible window to the brain. In this technical paper we provide a protocol that employs the mouse model of oxygen-induced retinopathy to study the mechanisms that lead to failure of vascular regeneration within the central nervous system after ischemic injury. The described system can also be harnessed to explore strategies to promote regrowth of functional blood vessels within the retina and CNS.
01:20
Vascular Resistance
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2024
Vascular resistance is a critical concept in understanding blood flow dynamics in the circulatory system. It refers to the resistance that blood encounters as it flows through the blood vessels. This resistance is a key factor in determining blood pressure and cardiac workload. The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...
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The two routes differ in their starting point and behavior. Vasculogenesis builds vessels from precursor cells, whereas angiogenesis uses existing vessels as the source for sprouting, migration, and remodeling. This distinction helps biologists determine whether a vascular network is being established initially or expanded and reorganized during development, repair, or tissue engineering.
Vascular endothelial growth factor and local oxygen conditions connect the tissue environment to vessel behavior. These signals can influence when vessels form, where they migrate, and how networks are remodeled. Studying them allows researchers to relate changes in tissue conditions to vascular responses rather than treating vessel formation as an isolated structural event.
Remodeling matters because a vascular network must be organized, not merely present. During angiogenesis, vessels may sprout and migrate before their arrangement is adjusted to the surrounding tissue. Examining this sequence helps explain how transport pathways become integrated with tissue needs, including oxygen and nutrient delivery and metabolic-waste removal.
Engineered tissues require functional blood-vessel networks to survive after implantation. Without an effective transport network, the tissue may not receive adequate oxygen and nutrients or remove metabolic waste. For this reason, controlling vascularization is central to tissue engineering and to designing constructs that can remain viable in the body.
Vascularization research shows how blood-vessel networks support biological organization beyond isolated vessel formation. In embryonic development, it helps clarify how transport networks arise and become arranged. In organs, studying these networks provides context for how oxygen and nutrient supply, along with waste removal, are connected to tissue function.
The same biological process can be examined in contrasting contexts. During wound repair, researchers can study how vessel formation and remodeling support damaged tissue. In tumor progression, they can investigate vascular changes associated with abnormal tissue growth. Comparing these settings helps distinguish shared vascular mechanisms from context-specific outcomes.
Evaluating only whether vessels appear may miss whether they form a useful transport system. Researchers also consider how vessels are organized and remodeled within the tissue. This broader view is relevant to embryonic development, wound repair, tumors, and engineered tissues because tissue survival and function depend on an integrated network.