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Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
JoVE Journal
Medicine
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JoVE Journal Medicine
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
DOI:

11:10 min

September 13, 2016

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Chapters

  • 00:00Title
  • 02:34Preparation: reagents
  • 03:14Preparation: operating table
  • 03:51Preparation: animal
  • 04:15Microfil injection: Portal venous system
  • 07:45Microfil injection: Hepatic venous system
  • 08:43Results: Micro CT reconstruction
  • 09:17Results: 3D color coded reconstructions of hepatic vascular trees and territories
  • 09:43Results: Serial sections and reconstruction

Summary

Automatic Translation

Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.

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