Ve-cadherin Staining
Ve-cadherin Staining - Related Videos
01:25
Structure of Cadherins
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2023
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
01:19
Cadherins in Tissue Organization
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2023
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues. Cell Sorting During Development Cell sorting plays an...
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
03:12
May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
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2023
This video describes the technique of staining bone marrow cells using May- Grünwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential counting.
10:55
Staining Proteins in Gels
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Cited by 7 •
2008
Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.
FAQs
The primary antibody provides molecular recognition by binding Ve-cadherin, whereas the labeled secondary antibody creates the detectable readout. Depending on the label, researchers observe either fluorescence or a chromogenic signal at locations containing the target protein. This two-antibody arrangement connects selective protein recognition with spatial visualization of endothelial cell-cell junctions.
The distribution and intensity of the signal provide complementary information about endothelial structure. A pattern concentrated at endothelial borders can be examined for junction formation and organization, while changes in signal intensity may accompany altered vascular integrity or experimental responses. These observations allow researchers to connect protein localization with the condition of the endothelial layer.
The method supports both fluorescent and chromogenic detection, so the choice depends on how the prepared cells or tissue will be examined. Fluorescence displays the labeled signal through microscopy, while chromogenic detection produces a visible color-based readout. Both approaches reveal Ve-cadherin distribution, allowing researchers to select the format suited to their imaging and analysis workflow.
Cells or tissue are first fixed and prepared so the sample can undergo antibody-based detection. A primary antibody is then applied to bind Ve-cadherin, followed by a labeled secondary antibody that generates the signal. The resulting staining pattern is examined to evaluate endothelial borders, junction formation, organization, and related vascular features.
Researchers examine changes in Ve-cadherin distribution and signal intensity to evaluate endothelial organization during angiogenesis or disease-associated changes. Because the method reveals cell-cell junctions, it can indicate how vascular structure and integrity respond under these conditions. This makes the staining pattern useful for comparing endothelial states across biological samples or experimental treatments.
In biology, the readout can support investigations of vascular development, endothelial barrier function, and responses to experimental treatments. Researchers can assess whether junctions form and remain organized, then relate those observations to vascular integrity. The approach therefore links a molecular adhesion marker with broader questions about blood vessel structure and endothelial behavior.