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Staining Proteins in Gels
JoVE Journal
Biology
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JoVE Journal Biology
Staining Proteins in Gels

Staining Proteins in Gels

DOI:
10.3791/760-v

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10:55 min

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July 08, 2008

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,
1Keck Graduate Institute of Applied Life Sciences,UVP, LLC, 2Proteomic Center,Keck Graduate Institute of Applied Life Sciences

Chapters

  • 00:00Title
  • 00:31Introduction
  • 00:55Coomassie Blue Staining
  • 02:49Silver Staining
  • 06:37Fluorescent Staining Using SYPRO Orange or Red
  • 09:08Fluorescent Protein Stain Using SYPRO Ruby
  • 10:15Conclusion

Summary

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Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.

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Staining ProteinsElectrophoretic MethodsCoomassie Blue StainingSilver StainingFluorescent StainingSYPRO OrangeSYPRO Ruby

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