Viral Rescue
Viral Rescue - Related Videos
04:46
A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation
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2011
We describe a technique to maintain oxygenation and ventilation using an endotracheal tube inserted nasally to the level of the naso-pharynx while sealing the mouth and nares for successful positive pressure ventilation.
04:38
A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome
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Cited by 4 •
2011
Capsulorhexis is an important step in phacoemulsification surgery. A surgeon creates a continous curvilinear tear on the anterior lens capsule by controlling the tearing vector forces. A peripherally extended tear is a serious complication. This video demonstrates a novel technique of rescuing capsular radial tear out using a cystotome.
03:06
Rescue of Infectious Virions from Cells Transfected with Bacterial Artificial Chromosome-Derived Synthetic RNA
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2026
Source: Yun, S. et al. Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses. J. Vis. Exp. (2015)This video demonstrates the recovery of infectious Japanese encephalitis virus from synthetic RNA transcribed from a bacterial artificial chromosome clone. It shows how cells are electroporated with the synthetic RNA, allowing the viral genome to direct the production of new virions. The resulting plaques formed in infected cell monolayers confirm...
07:35
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Cited by 15 •
2017
This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.
00:57
Viral Recombination
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2019
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment. Viral Recombination Can Create New Diseases Some diseases can infect multiple species. For example, pigs can be infected by some human and...
FAQs
Helper plasmids provide essential viral proteins that may not be supplied directly by the introduced genome. In permissive cells, these proteins work alongside cellular and viral enzymes to support genome replication, protein production, and assembly. Their inclusion can therefore enable recovery of infectious virus when the cloned DNA or complementary DNA genome alone is insufficient.
Permissive cells provide the biological environment needed for the introduced viral genetic material to function. They support genome replication, production of viral proteins, and assembly of new particles through the activity of cellular and viral enzymes. The suitability of these cells directly affects whether infectious virus can be recovered for later characterization.
Engineered mutations allow researchers to examine how specific genetic changes affect viral behavior. After recovery, the resulting virus can be characterized to investigate gene function, replication, host range, or pathogenic mechanisms. This approach connects a defined change in viral genetic material with measurable biological outcomes under appropriately controlled laboratory conditions.
Characterization of the recovered virus can reveal how its genetic features influence replication, host range, and pathogenic mechanisms. It can also help researchers examine gene function by comparing viruses carrying different genetic configurations. These outcomes make Viral Rescue a way to connect cloned viral sequences with experimentally observed properties in biological systems.
Viral Rescue supports several areas of virology, including the development of vaccine candidates and viral vectors, as well as investigations of antiviral strategies. Because researchers can begin with defined viral genetic material, the method enables controlled examination of viral properties and engineered changes that may be relevant to prevention, delivery systems, or treatment research.
Recovering infectious virus requires appropriate biosafety conditions because the laboratory process can produce biologically active particles. Controlled handling is especially important when researchers study engineered mutations or pathogenic mechanisms. Biosafety oversight helps ensure that investigations of replication, host range, vaccines, vectors, and antiviral strategies remain within suitable laboratory controls.