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1Département de biologie moléculaire, biochimie médicale et pathologie, Faculté de médecine,Centre de recherche sur le cancer de l’Université Laval, 2Oncology, Centre de recherche du CHU de Québec,Université Laval, 3Laboratoire d’études moléculaires des valvulopathies (LEMV), Groupe de recherche en valvulopathies (GRV),Quebec Heart and Lung Institute/Research Center, 4Department of Surgery,Université Laval
Chapters
- 00:05Title
- 00:49Plating HeLa-RFP-H2B Cells
- 03:38Adenovirus Transduction and Endogenous Protein Knockdown by siRNA Transfection in HeLa-RFP-H2B Cells Using CaPi Precipitates
- 07:46Live Cell Imaging of Mitotic Cells and Data Analysis
- 09:36Results: Adenofection Effectively Introduces Proteins of Interest without Affecting Mitosis
- 11:44Conclusion
We describe a method for depletion-rescue experiments that preserves cellular integrity and protein homeostasis. Adenofection enables functional analyses of proteins within biological processes that rely on finely tuned actin-based dynamics, such as mitotic cell division and myogenesis, at the single-cell level.
