Carbonic Anhydrase Ix Staining

Carbonic anhydrase IX (CAIX) staining is an immunohistochemical or immunofluorescent method used to identify CAIX-expressing cells in tissue samples, particularly in cancer research. It works by applying a specific antibody that binds CAIX on cell membranes, followed by a chromogenic or fluorescent detection system that reveals its distribution. Because hypoxia-inducible factor 1 activates CAIX expression under low-oxygen conditions, staining can map hypoxic regions and assess tumor biology. Researchers use it to characterize tumor microenvironments, support biomarker studies, and investigate links between hypoxia, extracellular pH regulation, invasion, and treatment response.

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JoVE Core - Biology

The Carbon Cycle

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2019

Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle. Biological Carbon Cycle All...

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JoVE Journal - Engineering
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Cited by 6 •

2017

A protocol for the parallel production of precipitated calcium carbonate and zeolitic material from blast furnace slag via mineral carbonation and alkaline hydrothermal conversion, respectively, is presented. The performance of the zeolitic material towards nickel adsorption is tested.

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2024

Atmospheric CO2 penetrates the concrete's pores and, in the presence of moisture, forms carbonic acid, which then reacts with calcium hydroxide in the hydrated cement, forming calcium carbonate. This process reduces the concrete's volume and is termed carbonation shrinkage. The concrete's permeability is slightly reduced as calcium carbonate produced during the reaction fills its pores. Furthermore, its strength is slightly enhanced as the water released during the reaction facilitates the...

Carbon Skeletons

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2026

Life on Earth is carbon-based, as all macromolecules that make up living organisms contain carbon atoms. All organic compounds have a carbon backbone. Each carbon atom is tetravalent and can bond with four other atoms, making it an extraordinarily flexible component of biological molecules. Because carbon’s valence electrons are stable, it rarely becomes an ion. As the carbon chain increases in length, structural modifications such as ring structures, double bonds, and branching side chains...

Microscopy and Staining: Gram, Capsule, and Endospore Staining

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2023

Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...

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