Colon Cancer Cell Exosomes
Colon Cancer Cell Exosomes - Related Videos
03:43
Exosome Block Preparation: A Protocol to Preserve Exosomes From Cultured Human Colon Cancer Cells
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2023
This video describes the technique of preparing exosome blocks from cultured gastrointestinal tumor cells. These exosome blocks can further be sliced, stained, and imaged to determine the position of specific proteins in the lipid bilayer vesicle.
04:21
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
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2023
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
03:18
Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants
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2023
In this video, we establish an ex vivo omental organ culture overlaid with GFP-expressing ovarian cancer cells to study ovarian cancer colonization within the omental explant. This protocol analyzes immune cell structures within the omentum known as milky spots that promote metastases.
08:49
Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
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Cited by 59 •
2016
This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.
28:15
Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
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Cited by 22 •
2010
Reduced/absent expression of Pms2 and/or ERCC1 in entire crypts is a frequent event within 10 cm on each side of colonic adenocarcinomas, likely the basis of a field defect with high mutability and progression to cancer. Deficiency in Ku86 or CcOI is much less frequent in these field defects.
FAQs
Their effects depend on a linked intracellular release sequence: endosomal membranes bud inward, intraluminal vesicles accumulate in multivesicular bodies, and those bodies fuse with the plasma membrane. This pathway matters because it makes tumor-derived molecular information available for uptake by surrounding or distant recipient cells, linking vesicle release to intercellular communication.
The molecular cargo carried by these vesicles provides different forms of information to recipient cells. Proteins, lipids, and nucleic acids can each contribute to changes in cellular responses, depending on which materials are packaged and delivered. Examining cargo therefore helps researchers connect vesicle-mediated communication with tumor behavior and changes in surrounding tissues.
Uptake patterns help indicate which recipient cells may receive tumor-derived molecular information and how broadly that information can spread. Differences in uptake can influence communication within the tumor microenvironment and may help explain effects on immune responses, invasion, or metastasis. Measuring these patterns therefore adds context to cargo analysis.
After recipient cells take up tumor-derived vesicles, their molecular cargo can alter communication between cancer cells and surrounding tissues. This signaling may shape the tumor microenvironment and influence immune responses, making exosomes relevant to studies of how tumors interact with nearby cells. Their effects also provide context for investigating invasion and metastasis.
Researchers can examine their presence in blood or other body fluids to investigate whether tumor-associated vesicles provide measurable information without directly sampling tumor tissue. Analysis of these fluids supports biomarker research by connecting circulating or accessible vesicles with colon cancer-related molecular information and disease-associated communication between cells.
Their study supports several complementary goals: clarifying communication between tumor cells and surrounding tissues, examining effects on the tumor microenvironment and immune responses, and investigating links to invasion and metastasis. Because their presence in body fluids also supports biomarker research, these vesicles are examined as potential treatment targets as well as sources of biological information.