Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Marco Giallombardo; Jorge Chac&#225;rtegui Borr&#225;s; Marta Castiglia; Nele Van Der Steen; Inge Mertens; Patrick Pauwels; Marc Peeters; Christian Rolfo

doi:10.3791/53900

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Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients’ Plasma Through qPCR: A Feasible Liquid Biopsy Tool

JoVE Journal
Medicine

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JoVE Journal Medicine

Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients’ Plasma Through qPCR: A Feasible Liquid Biopsy Tool

11,120 Views

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08:49 min

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May 27, 2016

DOI:

10.3791/53900-v

DOI:

10.3791/53900-v

•

08:49 min

May 27, 2016

•

11075 Views

Marco Giallombardo*^1,2, Jorge Chacártegui Borrás*^2,3, Marta Castiglia⁴, Nele Van Der Steen³, Inge Mertens^5,6, Patrick Pauwels^7,3, Marc Peeters^8,3, Christian Rolfo^2,3

¹Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics,University of Palermo ²Phase I-Early Clinical Trials Unit, Oncology Department,Antwerp University Hospital (UZA)³Center for Oncological Research (CORE),Antwerp University ⁴Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology,University of Palermo ⁵Flemish Institute for Technological Research (VITO)⁶CORE, Campus Groenenborger,Antwerp University ⁷Molecular Pathology, Pathology Department,Antwerp University Hospital (UZA)⁸Oncology Department,Antwerp University Hospital (UZA)

Summary

This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.