Murine Model Surgery
Murine Model Surgery - Related Videos
04:36
Murine Superficial Lymph Node Surgery
0 Views •
Cited by 10 •
2012
To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.
03:47
A Murine Model of Carotid Aneurysm Formation
0 Views •
2025
The present protocol describes a standardized surgical method for the induction of carotid aneurysms in a murine model through a single surgical operation.
07:32
Murine Flexor Tendon Injury and Repair Surgery
0 Views •
Cited by 45 •
2016
Flexor tendons in the hand are commonly injured, leading to impaired hand function. However, the scar-tissue healing response is not well characterized. A murine model of flexor tendon healing is demonstrated here. This model can enhance overall understanding of the healing process and assess therapeutic approaches to improve healing.
10:29
Principles of Rodent Surgery for the New Surgeon
0 Views •
Cited by 56 •
2011
Before attempting surgery, a new surgeon should have training in basic surgical techniques and concepts. This article will present basic surgical considerations with an emphasis on rodents.
04:35
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
0 Views •
2023
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
FAQs
Orthotopic tumors are placed in the tissue corresponding to the tumor’s native site, allowing the model to reflect that cancer’s local microenvironment more closely than a nonspecific implantation site. This context supports investigations of tumor growth, invasion, metastasis, and treatment response, making surgical placement important for biologically relevant cancer studies.
Consistent surgical technique reduces procedural variation between animals and experiments. Reproducible anesthesia, tissue handling, implantation, wound closure, and recovery monitoring help ensure that observed differences in tumor behavior or therapeutic response are less likely to result from inconsistent surgery. Standardization therefore strengthens comparisons across treatment groups and improves the translational relevance of preclinical findings.
These procedures can support several complementary cancer endpoints, including tumor growth, local invasion, metastatic behavior, and response to therapy. Depending on the experimental design, surgery may also create a setting for tumor resection or treatment delivery. Examining these outcomes in the same whole-organism model connects local tumor behavior with broader disease progression.
Anesthesia, aseptic conditions, careful tissue manipulation, secure wound closure, and postoperative monitoring are central safeguards. Anesthesia supports controlled procedures, while aseptic technique helps protect the surgical field. Monitoring recovery and animal welfare is essential after closure because the quality of recovery can influence both ethical oversight and the reliability of subsequent cancer measurements.
A typical workflow includes preparing the animal under anesthesia, maintaining aseptic conditions, making a targeted incision, manipulating the relevant tissue, and implanting tumor cells or grafts when required. Researchers then close the wound and monitor recovery. Each stage must be performed consistently so that later assessments of tumor development or treatment response remain interpretable.
Surgical approaches are useful when a study requires tumor removal, localized treatment delivery, or evaluation of disease after a defined intervention. They can also establish orthotopic tumors before monitoring growth, invasion, metastasis, or therapeutic response. In cancer research, this flexibility allows investigators to examine both disease development and the effects of clinically relevant procedures within a living organism.