Pcr Strip Tube

A PCR strip tube is a small, heat-resistant vessel designed to hold reaction mixtures during polymerase chain reaction (PCR), enabling controlled amplification of selected DNA sequences. In a thermal cycler, the tube withstands repeated heating and cooling steps that separate DNA strands, allow primers to bind target sequences, and support polymerase-driven synthesis of new strands. Strip tubes organize multiple reactions in parallel and can be sealed to reduce evaporation and contamination. In cancer research, they support assays that amplify tumor-associated mutations, pathogen sequences, or other genetic markers, contributing to molecular detection, genotyping, and validation of research findings.

Pcr Strip Tube - Related Videos

Research

JoVE EoE - PCR Techniques

Chip-in-a-Tube-Based Digital PCR for Quantification of Single Nucleotide Variants

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2025

This video demonstrates the utility of digital PCR in a chip-in-a-tube format for the detection of single nucleotide variations in DNA samples. A single PCR reaction is partitioned into chambers that act as independent PCR reactions, and the detection of fluorescence signals from the amplified targets in the chambers is used to compute the frequency of the variant allele in the sample.

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

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Cited by 5 •

2016

This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.

Education

JoVE Science Education - Clinical Skills

Tube Thoracostomy

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2023

Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Tube thoracostomy (chest tube placement) is a procedure during which a hollow tube is inserted into the thoracic cavity for drainage of fluid or air. Emergency chest tube insertion is performed for definitive treatment of tension pneumothorax, traumatic hemothorax, large-volume pleural effusions, and empyemas. Irrespective of the cause of air and fluid accumulation in the pleural space, the...

Determining the Antibacterial Efficacy of an Antibiotic-Loaded Polymeric Strip

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2025

Source: Sekar, A., et. al., A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials. J. Vis. Exp. (2023)This video demonstrates a luminescence-based assay for assessing the antibacterial efficacy of an antibiotic-loaded polymeric strip by quantifying ATP-driven luminescence, which decreases in bacterial cultures exposed to the antibiotic.

Voltammetry: Stripping Methods

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2024

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current. Anodic Stripping Voltammetry (ASV) ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...

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