3d Liver Model
3d Liver Model - Related Videos
09:27
The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat
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Cited by 11 •
2016
We describe a method to produce an animal model of liver fibrosis in the rat, and assess the degree of fibrosis by histological examination of the liver. The model can be used to study the development of liver disease as well as to test the efficacy of potential anti-fibrotic agents.
06:46
3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
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Cited by 3 •
2022
The present protocol optimizes the liver in situ perfusion/decellularization and two-photon microscopy methods to establish a reliable platform to visualize the dynamics of extracellular matrix (ECM) remodeling during non-alcoholic steatohepatitis (NASH).
09:17
3D Printing of Biomolecular Models for Research and Pedagogy
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Cited by 29 •
2017
Physical models of biomolecules can facilitate an understanding of their structure-function for the researcher, aid in communication between researchers, and serve as an educational tool in pedagogical endeavors. Here, we provide detailed guidance for the 3D printing of accurate models of biomolecules using fused filament fabrication desktop 3D printers.
11:43
Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones
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Cited by 6 •
2016
The ability of metastatic clones to colonize distant sites depends on their proliferation capacity and/or their ability to survive in the host microenvironment without significant proliferation. Here, we present an animal model that allows quantitative visualization of both types of liver colonization by metastatic clones.
06:26
Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease with Fibrosis
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Cited by 1 •
2025
Mouse model of metabolic dysfunction-associated steatotic liver disease (MASLD) with metabolic dysfunction, hepatic gene expression changes, and liver histopathological alterations that resemble human MASLD, including fibrosis that progresses to advanced fibrosis stage 3. This model can be used in studies of MASLD pathophysiology and in pre-clinical studies of new therapies.
FAQs
Cell arrangement within extracellular matrix scaffolds or other supportive materials enables liver cells, cancer cells, or organoids to establish cell-cell interactions and tissue organization. This three-dimensional structure also permits nutrient and oxygen gradients to develop, creating local conditions that can influence tumor behavior and make experimental responses more representative of liver tissue.
Nutrient and oxygen gradients reproduce differences that arise across an organized tissue environment rather than exposing every cell to identical culture conditions. These gradients can help investigators examine tumor growth, invasion, drug response, and treatment resistance under conditions that more closely resemble the tumor microenvironment. Their presence is a key advantage over simpler culture arrangements.
Conventional two-dimensional cultures provide fewer opportunities for cells to organize spatially and interact within a tissue-like environment. A 3D liver model supports cell-cell interactions, tissue organization, and gradients of nutrients and oxygen, allowing researchers to study cancer-related behavior in a setting that is more physiologically relevant than a flat culture surface.
In cancer research, these systems can be used to investigate tumor growth, invasion, liver metastasis, drug response, and treatment resistance. Because the model can include liver cells, cancer cells, or organoids, researchers can examine how malignant cells behave within an organized liver-related environment and evaluate responses in the context of surrounding tissue features.
A model may incorporate liver cells, cancer cells, or organoids arranged within an extracellular matrix scaffold or another supportive material. The selected components provide the basis for tissue organization and cell-cell interactions, while the three-dimensional arrangement allows nutrient and oxygen gradients to form. Together, these features create the experimental platform used for cancer-focused studies.
Researchers would use this approach when they need to study cancer behavior in conditions that better resemble the tumor microenvironment. It is particularly relevant for examining invasion, liver metastasis, drug response, and treatment resistance, where spatial organization and interactions among cells may provide information that conventional two-dimensional cultures do not capture as effectively.
These models provide a platform for testing drug responses and targeted therapies in an organized liver-related environment. Investigators can assess how cancer cells respond under tissue-like conditions that include cell interactions and nutrient or oxygen gradients. The resulting observations may help evaluate treatment resistance and improve the predictive value of preclinical studies.
Their broader value lies in connecting tissue organization with measurable cancer outcomes, including tumor growth, invasion, metastasis, treatment response, and resistance. By offering a more physiologically relevant setting than conventional two-dimensional cultures, these models may strengthen preclinical evaluation and help researchers study liver-associated cancer processes before moving toward further investigation.