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Immunology and Infection
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Retroviral Transduction of T-cell Receptors in Mouse T-cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Retroviral Transduction of T-cell Receptors in Mouse T-cells

Retroviral Transduction of T-cell Receptors in Mouse T-cells

DOI:
10.3791/2307-v

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10:14 min

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October 22, 2010

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, , ,
1NYU Cancer institute,New York University School of Medicine, 2Program in Structural Biology,New York University School of Medicine

Chapters

  • 00:00Title
  • 01:37Retroviral Transfection and T-cell Isolation
  • 03:55Prepapration of a Single-cell Suspension from Mouse Spleen
  • 04:51Isolation and Activation of Mouse T-cells
  • 06:27Infection of Activated T-cells
  • 08:21Expansion of Cells and Evaluation of Transduction Efficiency
  • 08:55Retroviral Expression of TCR on Mouse T-cells
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to produce antigen-specific mouse T-cells using retroviral transduction

Tags

Retroviral TransductionT-cell ReceptorsMouse T-cellsImmune SystemPeptide AntigensAntigen Presenting CellsFunctional OutcomesImmunology Related DiseasesTCR Transgenic Mouse ModelsBone Marrow ChimeraRetroviral Transduction MethodPeptide-MHC SpecificityDownstream ApplicationsHuman ImmunotherapyAdoptive TransferCancer Treatment

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