Subcutaneous Tumor Injection
Subcutaneous Tumor Injection - Related Videos
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
04:13
An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor
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2025
This video illustrates testing of adjuvant or additional therapy in a mouse model with incompletely resected subcutaneous tumors. The partially tumor-resected mouse is treated with an antibody targeting the anti-cytotoxic protein of T regulatory cells, potentially reducing tumor regrowth and suggesting effective adjuvant therapy.
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
03:52
Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model
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2025
This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.
04:10
Surgical Cisterna Magna Injection: A Method for Administering Tumor Cells Directly into Central Nervous System of Murine Model
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2023
In this video, we describe the procedure to deliver human-derived tumor cells into the cisterna magna of a mouse’s brain. The orthotopic mouse model can be used to study metastatic spread of cancer to the cranial meninges.
FAQs
Placement in subcutaneous tissue creates a localized site where tumor development can be followed as a discrete mass. Because the resulting tumor is accessible for repeated observation, researchers can track changes in size and progression under controlled experimental conditions. This makes the model useful for connecting treatment exposure with visible changes in tumor behavior over time.
The cell suspension provides the starting material for establishing the experimental tumor, while the surrounding subcutaneous tissue serves as the implantation site. Using a suitable medium helps researchers prepare tumor cells or tissue for introduction in a consistent form. Controlled conditions remain important because the model depends on reliable tumor establishment and measurable progression.
Measurements of tumor size and progression provide the primary longitudinal readouts in this model. Tissue analysis adds a complementary endpoint by allowing investigators to examine the tumor alongside external observations. Together, these findings can connect overall growth patterns with features identified in tumor tissue when studying cancer biology or treatment effects.
Controlled experimental conditions help researchers interpret differences in tumor growth or treatment response more confidently. When the model is established and monitored in a consistent setting, changes in tumor size or progression can be considered alongside the candidate therapy being studied. This supports structured comparisons of therapeutic strategies within an experimental cancer research system.
The workflow begins with preparing tumor cells or tissue in a suitable medium, followed by introduction into subcutaneous tissue. Once a localized tumor develops, researchers monitor its size and progression over time. Candidate therapies can then be evaluated through changes observed during follow-up, with tissue analysis used alongside these measurements to characterize study outcomes.
This model is useful when a study needs a measurable tumor mass for assessing candidate therapies. Researchers can use it to investigate tumor growth, compare treatment responses, and examine treatment safety within controlled experiments. Its accessible tumor location supports ongoing observation, making it practical for studies that require progression and response to be followed over time.
Treatment response is assessed by comparing tumor progression and size during follow-up with behavior observed under the experimental conditions. Changes in these measurements provide an indication of how a candidate therapy affects the localized tumor model. Tissue analysis can strengthen interpretation by supplying additional evidence about the tumor alongside the monitored response.
Within cancer research, this technique connects tumor biology with experimental treatment evaluation. The same system can support questions about how tumors grow, whether a candidate therapy changes that course, and what safety-related findings accompany treatment assessment. These uses make it a model for organizing measurable evidence about tumor progression and therapeutic response.