Surgical Injection
Surgical Injection - Related Videos
04:10
Surgical Cisterna Magna Injection: A Method for Administering Tumor Cells Directly into Central Nervous System of Murine Model
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2023
In this video, we describe the procedure to deliver human-derived tumor cells into the cisterna magna of a mouse’s brain. The orthotopic mouse model can be used to study metastatic spread of cancer to the cranial meninges.
03:48
Mouse Bladder Wall Injection
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Cited by 34 •
2011
Mouse bladder wall injection is a useful approach to orthotopically study bladder stem cell and cancer biology. This delicate microsurgical method can be mastered with careful technique and practice.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
09:19
Intraperitoneal Injection into Adult Zebrafish
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Cited by 238 •
2010
We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.
04:00
Generation of Mouse Proximal Colon Loop Model: A Surgical Procedure to Generate Proximal Colon Loop and Measure Intestinal Permeability by Detection of Fluorescent Markers in Serum Following Intraluminal Injection
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2025
In this video, we describe a surgical procedure to generate mouse proximal colon loop or pcLoop segment followed by intraluminal injection of fluorescent markers into the segment to study the intestinal epithelial permeability.
FAQs
Concentration, injection volume, tissue characteristics, and timing jointly shape where an agent travels and how long it remains. A higher or lower concentration can alter local exposure, while volume affects the area reached. Tissue properties influence movement and retention, and timing determines when the intervention is present relative to surgical and biological events. These variables help explain differences in response.
Needle or catheter placement determines whether the material reaches the intended anatomical site. Accurate positioning supports localized exposure and makes treatment-related effects easier to associate with the target tissue. If placement changes between experiments, distribution and retention may also change, complicating interpretation. For that reason, the delivery route and anatomical location are important experimental variables, not merely technical details.
By placing an agent at a defined site, researchers can examine changes in the tumor in relation to the intended local exposure. They can also evaluate whether effects extend into surrounding tissue, while the localized setup may limit exposure beyond the surgical area. This supports interpretation of tumor response, tissue response, and distribution as related but distinct observations.
A study plan should specify the agent, anatomical target, delivery method, concentration, injection volume, and timing during the operation. Researchers should also identify how they will evaluate distribution, retention, and biological response afterward. Defining these elements in advance helps connect the delivered intervention with observed changes and improves consistency when comparing experimental conditions.
Cancer researchers can apply this approach to evaluate localized drug delivery, administer agents in tumor models, introduce cells, or place biomaterials at a surgical site. These uses allow investigators to study how a targeted intervention affects tumor tissue and its immediate surroundings. The method is therefore relevant to experiments focused on local treatment effects rather than only generalized exposure.
Postoperative assessment can combine imaging with tissue analysis to examine treatment-related changes. Imaging may help evaluate the agent's distribution or retention, while tissue analysis can reveal biological responses in the tumor or surrounding tissue. Together, these outcomes help researchers determine whether the intervention reached the intended location and how the tissue responded to localized treatment.