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Journal / Biology / Intraperitoneal Injection into Adult Zebrafish
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Biology

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JoVE Journal Biology
Intraperitoneal Injection into Adult Zebrafish
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Intraperitoneal Injection into Adult Zebrafish

Article DOI: 10.3791/2126 • 09:20 min • August 30th, 2010
August 30th, 2010 •
1, 2, 2,3, 1
1Department of Organismal Biology and Anatomy, The University of Chicago, 2Committee on Molecular Metabolism and Nutrition, The University of Chicago, 3Department of Medicine, The University of Chicago

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We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.

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Intraperitoneal Injection Adult Zebrafish Reagent Absorption Metabolic Studies IP Injection Abdominal Cavity Pelvic Girdle Veterinary Methods Zero Mortality Bleeding At Injection Site Gentle Handling Fasting Weighing Anesthetizing Recovery Stress Minimization Small Injection Volume 35G Needle Cortland Salt Solution Osmotically Balanced Freshwater Fish Aeration Of Gills Surgical Plane Of Anesthesia
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