Intraperitoneal Injection into Adult Zebrafish

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Intraperitoneal Injection into Adult Zebrafish

DOI:

10.3791/2126-v

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09:19 min

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August 30, 2010

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Mary D. Kinkel, Stefani C. Eames, Louis H. Philipson³, Victoria E. Prince

¹Department of Organismal Biology and Anatomy,The University of Chicago, ²Committee on Molecular Metabolism and Nutrition,The University of Chicago, ³Department of Medicine,The University of Chicago

Chapters

00:00Title
00:54Introduction
01:19Pre-injection Preparations
05:34Cold-water Anesthesia, Injection, and Recovery
07:56Blood Glucose Levels After 72 Hour Fast and Glucose Injection
08:41Conclusion

Summary

Automatic Translation

We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.

Intraperitoneal Injection into Adult Zebrafish

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