Tumor Volume Assessment
Tumor Volume Assessment - Related Videos
04:13
A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies
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2025
This video demonstrates a technique to study the in vivo localization of tumor-specific antibodies in a mouse tumor xenograft model. Upon subcutaneously injecting cancer cells into mice for tumor formation, fluorescently-labeled antibodies are injected that bind to tumor-specific receptors, enabling in vivo assessment of antibody localization via imaging.
04:11
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
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2023
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
02:08
Bioluminescence Imaging to Assess Tumor Growth in an Organotypic Mouse Brain Slice
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2025
Source: Ciraku, L., et. al. An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth. J. Vis. Exp. (2021).This video demonstrates the imaging of luciferase-expressing tumor cells in an organotypic mouse brain slice. Luciferin is added to the medium, and the plate is placed inside the chamber of the in vivo imaging system. Parameters for image capture are adjusted, and the tumor's shape and size are defined. The bioluminescent signals emitted by the tumor...
03:13
An In Vitro Assay to Assess CAR T Cell Cytotoxicity against Tumor Spheroids
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2025
This video demonstrates an in vitro assay evaluating the cytotoxic effects of CAR T cells on 3D tumor spheroids expressing fluorescent proteins. This assessment utilizes the Annexin V red fluorescent probe, which binds to exposed phosphatidylserine residues on the outer leaflet of apoptotic cancer cells after interactions with CAR T cells. The red fluorescence emitted by the probe aids in distinguishing these cells from healthy ones emitting green fluorescence.
07:36
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
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2021
Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.
FAQs
Caliper readings of tumor length and width are converted into an ellipsoid-based volume estimate, allowing size to be represented by a single quantitative value. Because this calculation derives volume from limited dimensional measurements, consistent identification of the same tumor axes matters across time points. The resulting values support longitudinal comparisons rather than visual impressions alone.
Three-dimensional segmentation can quantify irregular tumors more effectively than measurements based only on length and width. By representing tumor shape throughout the imaged volume, this approach addresses cases in which an ellipsoid estimate may not adequately reflect the tumor’s geometry. It therefore expands volume assessment beyond caliper-based monitoring when imaging data are available.
Tumor growth curves organize repeated volume measurements over time, making changes in progression visible across treatment groups. Researchers can use these patterns to evaluate therapeutic efficacy and recognize responses that differ during the study. Changes in the trajectory may also provide evidence consistent with treatment resistance or later recurrence, supporting a more informative interpretation than a single measurement.
Standardized measurement procedures reduce avoidable differences between assessments and improve experimental reproducibility. Applying the same approach across time points and study groups makes volume estimates more suitable for comparison, particularly when investigators evaluate treatment effects. Consistency also strengthens interpretation of growth curves by helping distinguish biological changes from variation introduced by the measurement process.
A caliper-based workflow measures tumor length and width at repeated study time points, applies an ellipsoid-based calculation to those dimensions, and records the resulting estimates for longitudinal analysis. Maintaining a consistent procedure allows the measurements to be assembled into growth curves and compared among treatment groups, providing a practical monitoring approach in preclinical cancer research.
Serial volume measurements provide an objective way to track increasing tumor burden during an experiment. When tumor size becomes excessive, these data can help investigators apply humane endpoints rather than relying only on subjective observation. The same monitoring record can also document progression, treatment response, or recurrence while supporting more responsible study management.