Wharton’s Duct Clipping
Wharton’s Duct Clipping - Related Videos
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
04:11
Endolymphatic Duct Blockage as a Surgical Treatment Option for Ménière's Disease
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Cited by 1 •
2023
Endolymphatic duct blockage is a relatively new surgical method for patients suffering from Ménière's disease. Following a regular mastoidectomy, the endolymphatic duct is identified and ligated using a regular titanium hemoclip. The effectiveness of this procedure is currently being assessed in a randomized trial.
04:02
Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture
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2025
The integrated technique described in this protocol achieves real-time biliary mapping via indocyanine green (ICG) fluorescence, minimally invasive stone clearance under direct visualization, and secure primary duct repair (6-0 PDS), eliminating T-tube drainage.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
04:12
Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion
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2025
This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.
FAQs
The main effect of duct clipping is a sequence of pressure-related tissue stress followed by recognizable remodeling responses. As saliva flow is blocked, acinar-cell atrophy, inflammation, fibrosis, and altered epithelial organization can be evaluated as parts of the same injury model. This lets investigators relate mechanical obstruction to changes in glandular structure and cellular behavior.
The clip's removal creates a defined transition from injury to recovery. Researchers can compare tissue during obstruction with tissue after flow is restored, helping distinguish persistent remodeling from changes associated with repair or regeneration. That paired design is especially useful when studying whether epithelial organization and glandular structure can move toward a recovered state after a controlled insult.
Wharton’s Duct Clipping can create a controlled setting for studying how injury-associated inflammation, fibrosis, and altered epithelial organization shape the local glandular environment. In cancer research, this supports analysis of how tissue damage and repair may influence epithelial behavior and potentially contribute to disease development.
The core workflow has two analytical phases: first, examine the gland while the duct remains clipped; second, remove the clip and evaluate subsequent tissue changes. Comparing these phases allows each gland’s injury-associated state to be considered alongside its recovery response, rather than treating damage and repair as unrelated experiments.
Relevant readouts include acinar-cell atrophy, inflammation, fibrosis, and epithelial organization. Tracking these features across obstruction and recovery helps investigators determine whether a response is associated with tissue injury, remodeling, or regeneration. The resulting observations connect structural changes in the gland to broader questions about epithelial behavior.
The reversible model is useful for cancer studies because it links a defined injury state with a later recovery state. This enables investigators to ask whether injury-related changes persist, resolve, or reorganize after the clip is removed. Such comparisons help place epithelial behavior within a changing microenvironment rather than examining damage alone.