C18 Cartridge Purification
C18 Cartridge Purification - Related Videos
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
0 Views •
2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
05:43
A STAGE Tip Procedure for Desalting and Purification of Peptides
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2026
Source:Tia Rizakos1, Jennifer Geddes-McAlister11Molecular and Cellular Biology Department, University of Guelph.This video demonstrates peptide purification through the STAGE Tip procedure. A miniature column containing resin beads bonded to long hydrocarbon chains facilitates hydrophobic interaction-based purification. Upon loading a pre-digested peptide sample into the column, the peptide fragments bind to the resin beads, allowing impurities to pass through. An elution buffer that disrupts...
08:16
Plasmid Purification
0 Views •
2023
Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study. In this video, a step-by-step generalized procedure is described for how to perform plasmid purification.
06:30
Gel Purification
0 Views •
2023
Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, washing and eluting from a silica column. DNA is believed to bind to silica in the presence of high salt via a salt bridge. Following binding, DNA is washed of impurities and eluted under low salt conditions disrupting this interaction. This video goes through a step-by-step, generalized procedure for cutting out a band from the gel, gel...
FAQs
Retention arises from hydrophobic interactions between relatively nonpolar analytes and the octadecyl hydrocarbon chains bonded to the silica particles. More polar components interact less favorably with this nonpolar stationary phase under aqueous or mixed-solvent loading conditions, so they pass through instead of accumulating. This polarity-based partitioning provides the main source of cleanup selectivity.
Increasing the proportion of organic solvent weakens the preference of hydrophobic analytes for the bonded C18 phase. As the solvent environment becomes more favorable for those compounds, they leave the cartridge and enter the collected eluate. Controlling this change allows target compounds to be recovered after unwanted polar substances have been separated during loading.
The solvent composition determines whether analytes favor the mobile liquid or the hydrophobic cartridge phase. Aqueous or mixed-solvent conditions can promote retention of relatively nonpolar compounds while allowing polar components to pass through. Changing that composition later provides a practical way to alter selectivity and recover retained material without changing the cartridge itself.
A basic workflow begins by conditioning the cartridge, which prepares the bonded silica phase for sample contact. The sample is then loaded under aqueous or mixed-solvent conditions so suitable analytes can be retained. Finally, increasing the organic-solvent content elutes the target compounds. The collected eluate can then support a subsequent analytical procedure.
The technique is useful when a sample requires cleanup, desalting, or preconcentration before analysis. Removing unwanted polar components and concentrating retained compounds can produce a more suitable sample for high-performance liquid chromatography or mass spectrometry. In this role, the cartridge serves as a preparation step that improves the practical handling of complex chemical mixtures.
C18 cartridge purification has applications across synthetic, pharmaceutical, environmental, and biochemical chemistry. In each setting, its value comes from processing complex mixtures in a practical cartridge format while exploiting differences in compound polarity. The resulting cleanup or concentration step can support characterization, analytical measurements, or further chemical work involving the recovered compounds.