Hyperpolarized Nmr
Hyperpolarized Nmr - Related Videos
16:20
Hyperpolarized Xenon for NMR and MRI Applications
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Cited by 28 •
2012
The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are given.
09:05
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
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Cited by 1 •
2025
This protocol integrates a custom-built bioreactor system with hyperpolarized ¹³C NMR spectroscopy to measure real-time pyruvate metabolism in live cells, improving reproducibility and kinetic accuracy for metabolic studies and drug screening applications.
10:54
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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Cited by 14 •
2016
The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.
09:24
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
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Cited by 2 •
2017
A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical industry.
10:08
Nuclear Magnetic Resonance (NMR) Spectroscopy
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2023
Source: Laboratory of Dr. Henrik Sundén – Chalmers University of Technology Nuclear magnetic resonance (NMR) spectroscopy is a vital analysis technique for organic chemists. With the help of NMR, the work in the organic lab has been facilitated tremendously. Not only can it provide information about the structure of a molecule but also determine the content and purity of a sample. Compared with other commonly encountered techniques for organic chemists — such as thermal analysis and mass...
FAQs
Dynamic nuclear polarization transfers polarization from electron spins to nearby nuclear spins through microwave irradiation. The electron spins provide a strong initial polarization source, while the microwaves drive the transfer under the selected experimental conditions. This step produces a highly polarized sample before dissolution, supplying the enhanced starting signal needed for rapid chemical measurements.
Low-temperature conditions support the polarization stage by helping maintain the spin state before the sample is dissolved. Microwave irradiation is applied during this stage to promote transfer from electron spins to nuclei. Because the enhanced state is prepared before solution-phase observation, controlling the initial conditions is essential for obtaining a measurable signal after the sample enters the NMR experiment.
The enhanced polarization does not remain indefinitely after dissolution, so the observation window is limited. Measurements must begin rapidly and proceed before the signal enhancement decays substantially. This time constraint makes the technique especially useful for following fast chemical changes, while also requiring close coordination between dissolution, sample transfer, and NMR data collection.
Conventional NMR may not readily provide useful measurements for low-concentration compounds, insensitive nuclei, or rapidly changing systems. Hyperpolarized NMR addresses these limitations by starting with much stronger nuclear polarization, which improves signal intensity and can increase time resolution. The resulting measurements can reveal transient species and molecular transformations that would be difficult to observe otherwise.
A common workflow begins by polarizing the nuclei through dynamic nuclear polarization at low temperature with microwave irradiation. The polarized material is then rapidly dissolved, transferred into the observation environment, and monitored by NMR before the enhanced state decays. This sequence links sample preparation and measurement closely, because delays after dissolution reduce the available signal.
The approach is valuable when researchers need rapid reaction monitoring or information about short-lived chemical species. Enhanced signal intensity can make molecular transformations easier to follow as they occur, providing evidence about reaction behavior and mechanisms. It is therefore suited to dynamic chemical systems in which conventional measurements may lack sufficient sensitivity or time resolution.
By improving the detectability of low-concentration compounds and insensitive nuclei, the method can expose changes that are otherwise difficult to measure. Time-resolved observations may track reactants, products, or transient species during molecular transformations. In chemistry, these outcomes help connect observed signal changes with reaction mechanisms and the behavior of dynamic systems.