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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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JoVE Journal Chemistry
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

DOI:
10.3791/53548-v

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10:54 min

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February 23, 2016

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, , , , ,
1LCBPT – UMR8601, Institut de Chimie,Université Paris Descartes, 2Institute of Physics of Biological Systems,EPFL, 3PARCC – INSERM U970,Université Paris Descartes

Chapters

  • 00:05Title
  • 01:37Polarization
  • 03:29Solid-state NMR Measurements
  • 05:15Sample Preparation and Positioning
  • 06:19Dissolution and Injection
  • 07:32Liquid-state NMR Measurement
  • 08:25Results: Real-time Enzymatic Reaction Rate Measurement for LDH Activity
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

Tags

Artificial IntelligenceDynamic Nuclear PolarizationNMREnzymatic ReactionReal-time MeasurementDissolution DNPPolarizing SolutionCarbon-13TEMPO RadicalCryostatLiquid HeliumSample ContainerLiquid Nitrogen

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