Advance Directives

Advance directives are legal documents that record a person’s preferences for future medical care and identify who may make decisions if the person cannot communicate. They typically include a living will, which describes desired or declined interventions, and a healthcare proxy or durable power of attorney, which authorizes a surrogate decision-maker; these instructions guide clinicians when decision-making capacity is lost. In clinical practice, advance directives support patient autonomy, clarify goals of care, reduce uncertainty for families and healthcare teams, and inform decisions about life-sustaining treatment, resuscitation, and other serious medical interventions. Regular review helps keep documented preferences aligned with changing values and health circumstances.

Advance Directives - Related Videos

Research

JoVE Journal - Bioengineering

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

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Cited by 1 •

2024

Here, a protocol for designing, constructing, and delivering tetracycline-controlled based inducible synthetic circuit is presented. The effect of the synthetic circuits on parasite elimination and cytokine expression can be studied by channelizing the presented pipeline. This protocol is relevant for researchers studying synthetic circuit-based therapeutics in infectious diseases.

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

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Cited by 1 •

2016

Here we present a reliable method to monitor the incorporation of nanoparticles into a polymer host matrix via swell encapsulation. We show that the surface concentration of cadmium selenide quantum dots can be accurately visualized through cross-sectional fluorescence imaging.

Education

JoVE Core - Analytical Chemistry

Extraction: Advanced Methods

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2024

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

Directing Effect of Substituents: meta-Directing Groups

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2025

Substituents on the benzene ring that direct an incoming electrophile to undergo substitution at the meta position are called meta directors. All meta directors either have a positive charge on the atom directly bonded to the ring or a partial positive charge. These groups function by withdrawing electrons from the ring through inductive and resonance effects. Consider the carbocation intermediates formed upon the addition of an electrophile on nitrobenzene at the ortho, meta, and para...

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

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Cited by 6 •

2022

Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.

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